We developed a micropatterned photodegradable hydrogel array integrated with reconfigurable microfluidics to enable cell secretion analysis and cell retrieval at the single-cell level. The activity of protease molecules secreted from single cells was monitored using FRET peptides entrapped inside microfabricated compartments. Antibody-modified gel islands tethering cells to the surface could be degraded by UV exposure to release specific single cells of interest.
|Original language||English (US)|
|Number of pages||5|
|Journal||Lab on a Chip - Miniaturisation for Chemistry and Biology|
|State||Published - Feb 7 2015|
ASJC Scopus subject areas
- Biomedical Engineering