A microsystem integrating photodegradable hydrogel microstructures and reconfigurable microfluidics for single-cell analysis and retrieval

Kyung Jin Son, Dong Sik Shin, Timothy Kwa, Jungmok You, Yandong Gao, Alexander Revzin

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We developed a micropatterned photodegradable hydrogel array integrated with reconfigurable microfluidics to enable cell secretion analysis and cell retrieval at the single-cell level. The activity of protease molecules secreted from single cells was monitored using FRET peptides entrapped inside microfabricated compartments. Antibody-modified gel islands tethering cells to the surface could be degraded by UV exposure to release specific single cells of interest.

Original languageEnglish (US)
Pages (from-to)637-641
Number of pages5
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume15
Issue number3
DOIs
StatePublished - Feb 7 2015

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

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