A microfluidic platform to study astrocyte adhesion on nanoporous gold thin films

Alexander E. Hampe, Zidong Li, Sunjay Sethi, Pamela J Lein, Erkin Seker

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Nanoporous gold (np-Au) electrode coatings have shown improved neural electrophysiological recording fidelity in vitro, in part due to reduced surface coverage by astrocytes. This reduction in astrocytic spreading has been attributed to the influence of electrode nanostructure on focal adhesion (FA) formation. This study describes the development and use of a microfluidic flow cell for imposing controllable hydrodynamic shear on astrocytes cultured on gold surfaces of different morphologies, in order to study the influence of nanostructure on astrocyte adhesion strength as a function of np-Au electrode morphology. Astrocyte detachment (a surrogate for adhesion strength) monotonically increased as feature size was reduced from planar surfaces to np-Au, demonstrating that adhesion strength is dependent on nanostructure. Putative mechanisms responsible for this nanostructure-driven detachment phenomenon are also discussed.

Original languageEnglish (US)
Article number452
JournalNanomaterials
Volume8
Issue number7
DOIs
StatePublished - Jul 1 2018

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Keywords

  • Adhesion strength
  • Astrocyte
  • Cell-material interaction
  • Focal adhesion
  • Microfluidic flow-cell
  • Nanoporous gold
  • Nanostructure

ASJC Scopus subject areas

  • Materials Science(all)
  • Chemical Engineering(all)

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