A method to estimate mitochondrial Ca2+ uptake in intact cardiac myocytes

J. W M Bassani, R. A. Bassani, Donald M Bers

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4 Scopus citations


In the present paper we describe a method to estimate mitochondrial Ca2+ uptake during the declining phase of Ca2+ transients (cell relaxation) in intact isolated myocardial cells. This method is based on inhibition of sarcoplasmic reticulum (SR) Ca2+ accumulation by caffeine, blockade of Ca2+ transport via sarcolemmal Ca2+-ATPase by treatment with carboxyeosin and inhibition of sarcolemmal Na+/Ca2+ exchange by removal of extracellular Na+ and Ca2+. Ca2+ transients were evoked in rabbit ventricular myocytes by quick and sustained caffeine application (10 mM) after a 5-min period of electrical stimulation to load the SR with Ca2+. Mitochondrial Ca2+ transport was estimated using a model described by Sipido and Wier (Journal of Physiology (1991), 435: 605-630), which was originally proposed to describe Ca2+ fluxes during excitation-contraction coupling in cardiac cells. Our results indicate that, in intact rabbit myocytes, the Ca2+ flux due to net mitochondrial Ca2+ uptake may attain a value close to 1 μM/ sec.

Original languageEnglish (US)
Pages (from-to)1699-1707
Number of pages9
JournalBrazilian Journal of Medical and Biological Research
Issue number12
StatePublished - 1996
Externally publishedYes


  • Ca-atpase
  • Cardiac myocytes
  • Cytosolic Ca
  • Mitochondrial Ca uniporter
  • Na/Ca exchange
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Medicine (miscellaneous)


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