A mechanism for acetylcholine receptor clustering distinct from agrin signaling

Wade A. Grow, Michael J Ferns, Herman Gordon

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Acetylcholine receptors (AChRs) and other postsynaptic molecules cluster spontaneously on cultured C2 myotubes. The frequency of clustering is enhanced by neural agrin, neuraminidase, or calcium through a signaling pathway which includes tyrosine phosphorylation of a muscle-specific kinase (MuSK) and the AChR β-subunit Vicia villosa agglutinin (VVA) lectin, previously shown to potentiate agrin-induced clustering on C2 myotubes, is shown here to also potentiate neuraminidase- and calcium-induced clustering of AChRs, while having no effect on the level of tyrosine phosphorylation of MuSK or the AChR β-subunit. We propose that VVA lectin increases the frequency of AChR clustering through a mechanism that is distinct from agrin signaling, and that may involve α-dystroglycan. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish (US)
Pages (from-to)435-443
Number of pages9
JournalDevelopmental Neuroscience
Issue number6
StatePublished - 2000
Externally publishedYes


  • Acetylcholine receptor
  • Agrin
  • Laminin
  • Myotubes
  • Neuraminidase
  • Sialic acid
  • Vicia villosa agglutinin lectin
  • α-Dystroglycan

ASJC Scopus subject areas

  • Neuroscience(all)


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