A mechanism for acetylcholine receptor clustering distinct from agrin signaling

Wade A. Grow, Michael J Ferns, Herman Gordon

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Acetylcholine receptors (AChRs) and other postsynaptic molecules cluster spontaneously on cultured C2 myotubes. The frequency of clustering is enhanced by neural agrin, neuraminidase, or calcium through a signaling pathway which includes tyrosine phosphorylation of a muscle-specific kinase (MuSK) and the AChR β-subunit Vicia villosa agglutinin (VVA) lectin, previously shown to potentiate agrin-induced clustering on C2 myotubes, is shown here to also potentiate neuraminidase- and calcium-induced clustering of AChRs, while having no effect on the level of tyrosine phosphorylation of MuSK or the AChR β-subunit. We propose that VVA lectin increases the frequency of AChR clustering through a mechanism that is distinct from agrin signaling, and that may involve α-dystroglycan. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish (US)
Pages (from-to)435-443
Number of pages9
JournalDevelopmental Neuroscience
Volume21
Issue number6
StatePublished - 2000
Externally publishedYes

Keywords

  • Acetylcholine receptor
  • Agrin
  • Laminin
  • Myotubes
  • Neuraminidase
  • Sialic acid
  • Vicia villosa agglutinin lectin
  • α-Dystroglycan

ASJC Scopus subject areas

  • Neuroscience(all)

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