A mammalian cell mutant with a temperature sensitive leucyl transfer RNA synthetase

L. H. Thompson, J. L. Harkins, C. P. Stanners

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133 Scopus citations


A cell mutant of the Chinese hamster ovary line, which is temperature sensitive for protein synthesis, is specifically defective in vivo in its ability to charge tRNA with leucine. Cytoplasmic extracts exhibited temperature sensitive leucyl tRNA synthetase activity. It is, therefore, highly likely that the mutant has a structural alteration in leucyl tRNA synthetase. The low leakiness and low reversion rate of this mutant, combined with the specificity of the defect in its protein synthesizing machinery, make it an appealing tool for investigating regulatory mechanisms in animal cells.

Original languageEnglish (US)
Pages (from-to)3094-3098
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number11
StatePublished - 1973
Externally publishedYes


ASJC Scopus subject areas

  • General
  • Genetics

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