A magnetic-bead-based mosquito DNA extraction protocol for next-generation sequencing

Tse Yu Chen, Adam E. Vorsino, Kyle J. Kosinski, Ana L. Romero-Weaver, Eva A. Buckner, Joanna C. Chiu, Yoosook Lee

Research output: Contribution to journalArticlepeer-review

Abstract

A recently published DNA extraction protocol using magnetic beads and an automated DNA extraction instrument suggested that it is possible to extract high quality and quantity DNA from a well-preserved individual mosquito sufficient for downstream whole genome sequencing. However, reliance on an expensive automated DNA extraction instrument can be prohibitive for many laboratories. Here, the study provides a budget-friendly magnetic-bead-based DNA extraction protocol, which is suitable for low to medium throughput. The protocol described here was successfully tested using individual Aedes aegypti mosquito samples. The reduced costs associated with high quality DNA extraction will increase the application of high throughput sequencing to resource limited labs and studies.

Original languageEnglish (US)
Article numbere62354
JournalJournal of Visualized Experiments
Volume2021
Issue number170
DOIs
StatePublished - 2021
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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