A lytic viral long noncoding RNA modulates the function of a latent protein

Mel Campbell, Kevin Y. Kim, Pei Ching Chang, Steve Huerta, Bogdan Shevchenko, Don Hong Wang, Chie Izumiya, Hsing Jien Kung, Yoshihiro Izumiya

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Latent Kaposi's sarcoma-associated herpesvirus (KSHV) episomes are coated with viral latency-associated nuclear antigen (LANA). In contrast, LANA rapidly disassociates from episomes during reactivation. Lytic KSHV expresses polyadenylated nuclear RNA (PAN RNA), a long noncoding RNA (lncRNA). We report that PAN RNA promotes LANA-episome disassociation through an interaction with LANA which facilitates LANA sequestration away from KSHV episomes during reactivation. These findings suggest that KSHV may have evolved an RNA aptamer to regulate latent protein function.

Original languageEnglish (US)
Pages (from-to)1843-1848
Number of pages6
JournalJournal of Virology
Volume88
Issue number3
DOIs
StatePublished - Feb 2014

Fingerprint

nuclear antigens
Long Noncoding RNA
Human herpesvirus 8
Human Herpesvirus 8
plasmids
Plasmids
Nuclear RNA
RNA
Proteins
proteins
Nucleotide Aptamers
Virus Latency
Messenger RNA
latency-associated nuclear antigen
non-coding RNA

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

A lytic viral long noncoding RNA modulates the function of a latent protein. / Campbell, Mel; Kim, Kevin Y.; Chang, Pei Ching; Huerta, Steve; Shevchenko, Bogdan; Wang, Don Hong; Izumiya, Chie; Kung, Hsing Jien; Izumiya, Yoshihiro.

In: Journal of Virology, Vol. 88, No. 3, 02.2014, p. 1843-1848.

Research output: Contribution to journalArticle

Campbell, M, Kim, KY, Chang, PC, Huerta, S, Shevchenko, B, Wang, DH, Izumiya, C, Kung, HJ & Izumiya, Y 2014, 'A lytic viral long noncoding RNA modulates the function of a latent protein', Journal of Virology, vol. 88, no. 3, pp. 1843-1848. https://doi.org/10.1128/JVI.03251-13
Campbell, Mel ; Kim, Kevin Y. ; Chang, Pei Ching ; Huerta, Steve ; Shevchenko, Bogdan ; Wang, Don Hong ; Izumiya, Chie ; Kung, Hsing Jien ; Izumiya, Yoshihiro. / A lytic viral long noncoding RNA modulates the function of a latent protein. In: Journal of Virology. 2014 ; Vol. 88, No. 3. pp. 1843-1848.
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