A lytic viral long noncoding RNA modulates the function of a latent protein

Mel Campbell, Kevin Y. Kim, Pei Ching Chang, Steve Huerta, Bogdan Shevchenko, Don Hong Wang, Chie Izumiya, Hsing-Jien Kung, Yoshihiro Izumiya

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Latent Kaposi's sarcoma-associated herpesvirus (KSHV) episomes are coated with viral latency-associated nuclear antigen (LANA). In contrast, LANA rapidly disassociates from episomes during reactivation. Lytic KSHV expresses polyadenylated nuclear RNA (PAN RNA), a long noncoding RNA (lncRNA). We report that PAN RNA promotes LANA-episome disassociation through an interaction with LANA which facilitates LANA sequestration away from KSHV episomes during reactivation. These findings suggest that KSHV may have evolved an RNA aptamer to regulate latent protein function.

Original languageEnglish (US)
Pages (from-to)1843-1848
Number of pages6
JournalJournal of Virology
Volume88
Issue number3
DOIs
StatePublished - Feb 2014

Fingerprint

nuclear antigens
Long Noncoding RNA
Human herpesvirus 8
Human Herpesvirus 8
plasmids
Plasmids
Nuclear RNA
RNA
Proteins
proteins
Nucleotide Aptamers
Virus Latency
Messenger RNA
latency-associated nuclear antigen
non-coding RNA

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Campbell, M., Kim, K. Y., Chang, P. C., Huerta, S., Shevchenko, B., Wang, D. H., ... Izumiya, Y. (2014). A lytic viral long noncoding RNA modulates the function of a latent protein. Journal of Virology, 88(3), 1843-1848. https://doi.org/10.1128/JVI.03251-13

A lytic viral long noncoding RNA modulates the function of a latent protein. / Campbell, Mel; Kim, Kevin Y.; Chang, Pei Ching; Huerta, Steve; Shevchenko, Bogdan; Wang, Don Hong; Izumiya, Chie; Kung, Hsing-Jien; Izumiya, Yoshihiro.

In: Journal of Virology, Vol. 88, No. 3, 02.2014, p. 1843-1848.

Research output: Contribution to journalArticle

Campbell, M, Kim, KY, Chang, PC, Huerta, S, Shevchenko, B, Wang, DH, Izumiya, C, Kung, H-J & Izumiya, Y 2014, 'A lytic viral long noncoding RNA modulates the function of a latent protein', Journal of Virology, vol. 88, no. 3, pp. 1843-1848. https://doi.org/10.1128/JVI.03251-13
Campbell M, Kim KY, Chang PC, Huerta S, Shevchenko B, Wang DH et al. A lytic viral long noncoding RNA modulates the function of a latent protein. Journal of Virology. 2014 Feb;88(3):1843-1848. https://doi.org/10.1128/JVI.03251-13
Campbell, Mel ; Kim, Kevin Y. ; Chang, Pei Ching ; Huerta, Steve ; Shevchenko, Bogdan ; Wang, Don Hong ; Izumiya, Chie ; Kung, Hsing-Jien ; Izumiya, Yoshihiro. / A lytic viral long noncoding RNA modulates the function of a latent protein. In: Journal of Virology. 2014 ; Vol. 88, No. 3. pp. 1843-1848.
@article{eb72179d107b4640bba82d33d24c8987,
title = "A lytic viral long noncoding RNA modulates the function of a latent protein",
abstract = "Latent Kaposi's sarcoma-associated herpesvirus (KSHV) episomes are coated with viral latency-associated nuclear antigen (LANA). In contrast, LANA rapidly disassociates from episomes during reactivation. Lytic KSHV expresses polyadenylated nuclear RNA (PAN RNA), a long noncoding RNA (lncRNA). We report that PAN RNA promotes LANA-episome disassociation through an interaction with LANA which facilitates LANA sequestration away from KSHV episomes during reactivation. These findings suggest that KSHV may have evolved an RNA aptamer to regulate latent protein function.",
author = "Mel Campbell and Kim, {Kevin Y.} and Chang, {Pei Ching} and Steve Huerta and Bogdan Shevchenko and Wang, {Don Hong} and Chie Izumiya and Hsing-Jien Kung and Yoshihiro Izumiya",
year = "2014",
month = "2",
doi = "10.1128/JVI.03251-13",
language = "English (US)",
volume = "88",
pages = "1843--1848",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - A lytic viral long noncoding RNA modulates the function of a latent protein

AU - Campbell, Mel

AU - Kim, Kevin Y.

AU - Chang, Pei Ching

AU - Huerta, Steve

AU - Shevchenko, Bogdan

AU - Wang, Don Hong

AU - Izumiya, Chie

AU - Kung, Hsing-Jien

AU - Izumiya, Yoshihiro

PY - 2014/2

Y1 - 2014/2

N2 - Latent Kaposi's sarcoma-associated herpesvirus (KSHV) episomes are coated with viral latency-associated nuclear antigen (LANA). In contrast, LANA rapidly disassociates from episomes during reactivation. Lytic KSHV expresses polyadenylated nuclear RNA (PAN RNA), a long noncoding RNA (lncRNA). We report that PAN RNA promotes LANA-episome disassociation through an interaction with LANA which facilitates LANA sequestration away from KSHV episomes during reactivation. These findings suggest that KSHV may have evolved an RNA aptamer to regulate latent protein function.

AB - Latent Kaposi's sarcoma-associated herpesvirus (KSHV) episomes are coated with viral latency-associated nuclear antigen (LANA). In contrast, LANA rapidly disassociates from episomes during reactivation. Lytic KSHV expresses polyadenylated nuclear RNA (PAN RNA), a long noncoding RNA (lncRNA). We report that PAN RNA promotes LANA-episome disassociation through an interaction with LANA which facilitates LANA sequestration away from KSHV episomes during reactivation. These findings suggest that KSHV may have evolved an RNA aptamer to regulate latent protein function.

UR - http://www.scopus.com/inward/record.url?scp=84892457253&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892457253&partnerID=8YFLogxK

U2 - 10.1128/JVI.03251-13

DO - 10.1128/JVI.03251-13

M3 - Article

C2 - 24257619

AN - SCOPUS:84892457253

VL - 88

SP - 1843

EP - 1848

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 3

ER -