A lymphocytotoxic factor(s) in plasma of patients with minimal change nephrotic syndrome: Partial characterization

B. P. Barna, Sudesh P Makker, R. Kallen, R. Valenzuela, S. D. Deodhar, M. Yeip, D. Leto, M. A. Verbic, S. Rajaraman, S. Govindarajan

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Abstract

The incidence, relationship to clinical disease, and physical characteristics of a plasma cytotoxic factor(s) were studied in steroid-responsive minimal change nephrotic syndrome (MCNS) and other renal diseases. Plasma activity was found in 76% of 67 children with MCNS and in 67% of 9 children with focal segmental sclerosis (FSS). Of 31 normal adults and children and 7 adults with membranous glomerulonephritis, only 1 individual had toxic plasma. In MCNS, degree of plasma activity was not related to clinical disease, prednisone dosage, or serum levels of IgG or α-2-macroglobulin. The active factor(s) was found more frequently in plasma than in serum, was heat stable and nondialyzable by selected filtration, and was approximately 100,000 to 300,000 molecular weight. By DEAE column chromatography, activity coincided with fractions containing IgA and IgM but not IgG. While the nature of the plasma factor(s) has not been identified, these data indicate that MCNS plasma may adversely affect lymphocyte viability by a slow process of cytotoxicity requiring 24 or more hr, and that such plasma activity occurs frequently in children with MCNS and also with the more severe FSS.

Original languageEnglish (US)
Pages (from-to)272-282
Number of pages11
JournalClinical Immunology and Immunopathology
Volume27
Issue number2
DOIs
StatePublished - 1983
Externally publishedYes

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Lipoid Nephrosis
Sclerosis
Immunoglobulin G
Macroglobulins
Membranous Glomerulonephritis
Poisons
Prednisone
Serum
Immunoglobulin A
Immunoglobulin M
Chromatography
Hot Temperature
Molecular Weight
Steroids
Lymphocytes
Kidney
Incidence

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

A lymphocytotoxic factor(s) in plasma of patients with minimal change nephrotic syndrome : Partial characterization. / Barna, B. P.; Makker, Sudesh P; Kallen, R.; Valenzuela, R.; Deodhar, S. D.; Yeip, M.; Leto, D.; Verbic, M. A.; Rajaraman, S.; Govindarajan, S.

In: Clinical Immunology and Immunopathology, Vol. 27, No. 2, 1983, p. 272-282.

Research output: Contribution to journalArticle

Barna, BP, Makker, SP, Kallen, R, Valenzuela, R, Deodhar, SD, Yeip, M, Leto, D, Verbic, MA, Rajaraman, S & Govindarajan, S 1983, 'A lymphocytotoxic factor(s) in plasma of patients with minimal change nephrotic syndrome: Partial characterization', Clinical Immunology and Immunopathology, vol. 27, no. 2, pp. 272-282. https://doi.org/10.1016/0090-1229(83)90077-6
Barna, B. P. ; Makker, Sudesh P ; Kallen, R. ; Valenzuela, R. ; Deodhar, S. D. ; Yeip, M. ; Leto, D. ; Verbic, M. A. ; Rajaraman, S. ; Govindarajan, S. / A lymphocytotoxic factor(s) in plasma of patients with minimal change nephrotic syndrome : Partial characterization. In: Clinical Immunology and Immunopathology. 1983 ; Vol. 27, No. 2. pp. 272-282.
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