Abstract
Objective: To determine whether reactive oxygen metabolite production by alveolar macrophages is affected by liquid perfluorochemical exposure. Design: Controlled, animal laboratory investigation of alveolar macrophage function in vitro. Setting: Animal research facility of a health sciences university. Subjects: Six adult male New Zealand white rabbits and six young piglets. Interventions: Alveolar macrophages were obtained after sacrifice from both species by total lung lavage. Macrophages were divided into control and experimental groups. Macrophages in the experimental groups were exposed to perfluorooctylbromide. To determine production of reactive oxygen metabolites, hydrogen peroxide production and chemiluminescence were measured in both experimental and control groups after chemical stimulation. Measurements and Main Results: Perfluorooctylbromide-exposed alveolar macrophages produced significantly less hydrogen peroxide (1.4 ± 1.5 vs. 2.4 ± 1.6 nmol/106 cells; p = .002). Perfluorooctylbromide-exposed alveolar macrophages demonstrated significantly less chemiluminescence activity compared with nonexposed cells (0.70 ± 0.2 vs. 1.5 ± 0.2 mV of relative activity per 3.5 x 105 cells; p = .005). Conclusions: Exposure of alveolar macrophages to perfluorooctylbromide in vitro decreases the responsiveness of macrophages to potent stimuli. This finding may partially explain the decrease in pulmonary inflammation seen in animals treated with partial liquid ventilation during experimentally induced lung injury.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1533-1539 |
| Number of pages | 7 |
| Journal | Critical Care Medicine |
| Volume | 23 |
| Issue number | 9 |
| DOIs | |
| State | Published - 1995 |
| Externally published | Yes |
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Keywords
- alveolar macrophage
- chemiluminescence
- hydrogen peroxide
- inflammation
- lung
- perfluorocarbons
- reactive oxygen species
- superoxide anion
ASJC Scopus subject areas
- Critical Care and Intensive Care Medicine
Cite this
A liquid perfluorochemical decreases the in vitro production of reactive oxygen species by alveolar macrophages. / Smith, T. M.; Steinhorn, D. M.; Thusu, K.; Fuhrman, B. P.; Dandona, P.
In: Critical Care Medicine, Vol. 23, No. 9, 1995, p. 1533-1539.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A liquid perfluorochemical decreases the in vitro production of reactive oxygen species by alveolar macrophages
AU - Smith, T. M.
AU - Steinhorn, D. M.
AU - Thusu, K.
AU - Fuhrman, B. P.
AU - Dandona, P.
PY - 1995
Y1 - 1995
N2 - Objective: To determine whether reactive oxygen metabolite production by alveolar macrophages is affected by liquid perfluorochemical exposure. Design: Controlled, animal laboratory investigation of alveolar macrophage function in vitro. Setting: Animal research facility of a health sciences university. Subjects: Six adult male New Zealand white rabbits and six young piglets. Interventions: Alveolar macrophages were obtained after sacrifice from both species by total lung lavage. Macrophages were divided into control and experimental groups. Macrophages in the experimental groups were exposed to perfluorooctylbromide. To determine production of reactive oxygen metabolites, hydrogen peroxide production and chemiluminescence were measured in both experimental and control groups after chemical stimulation. Measurements and Main Results: Perfluorooctylbromide-exposed alveolar macrophages produced significantly less hydrogen peroxide (1.4 ± 1.5 vs. 2.4 ± 1.6 nmol/106 cells; p = .002). Perfluorooctylbromide-exposed alveolar macrophages demonstrated significantly less chemiluminescence activity compared with nonexposed cells (0.70 ± 0.2 vs. 1.5 ± 0.2 mV of relative activity per 3.5 x 105 cells; p = .005). Conclusions: Exposure of alveolar macrophages to perfluorooctylbromide in vitro decreases the responsiveness of macrophages to potent stimuli. This finding may partially explain the decrease in pulmonary inflammation seen in animals treated with partial liquid ventilation during experimentally induced lung injury.
AB - Objective: To determine whether reactive oxygen metabolite production by alveolar macrophages is affected by liquid perfluorochemical exposure. Design: Controlled, animal laboratory investigation of alveolar macrophage function in vitro. Setting: Animal research facility of a health sciences university. Subjects: Six adult male New Zealand white rabbits and six young piglets. Interventions: Alveolar macrophages were obtained after sacrifice from both species by total lung lavage. Macrophages were divided into control and experimental groups. Macrophages in the experimental groups were exposed to perfluorooctylbromide. To determine production of reactive oxygen metabolites, hydrogen peroxide production and chemiluminescence were measured in both experimental and control groups after chemical stimulation. Measurements and Main Results: Perfluorooctylbromide-exposed alveolar macrophages produced significantly less hydrogen peroxide (1.4 ± 1.5 vs. 2.4 ± 1.6 nmol/106 cells; p = .002). Perfluorooctylbromide-exposed alveolar macrophages demonstrated significantly less chemiluminescence activity compared with nonexposed cells (0.70 ± 0.2 vs. 1.5 ± 0.2 mV of relative activity per 3.5 x 105 cells; p = .005). Conclusions: Exposure of alveolar macrophages to perfluorooctylbromide in vitro decreases the responsiveness of macrophages to potent stimuli. This finding may partially explain the decrease in pulmonary inflammation seen in animals treated with partial liquid ventilation during experimentally induced lung injury.
KW - alveolar macrophage
KW - chemiluminescence
KW - hydrogen peroxide
KW - inflammation
KW - lung
KW - perfluorocarbons
KW - reactive oxygen species
KW - superoxide anion
UR - http://www.scopus.com/inward/record.url?scp=0029155972&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029155972&partnerID=8YFLogxK
U2 - 10.1097/00003246-199509000-00014
DO - 10.1097/00003246-199509000-00014
M3 - Article
C2 - 7664556
AN - SCOPUS:0029155972
VL - 23
SP - 1533
EP - 1539
JO - Critical Care Medicine
JF - Critical Care Medicine
SN - 0090-3493
IS - 9
ER -