A large-scale screen for mutagen-sensitive loci in Drosophila

Anne Laurencon, Charisse M. Orme, Heather K. Peters, Christina L. Boulton, Eszter K. Vladar, Sasha A. Langley, Emmanuel P. Bakis, David T. Harris, Nathan J. Harris, Sarah M. Wayson, R. Scott Hawley, Kenneth C. Burtis

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In a screen for new DNA repair mutants, we tested 6275 Drosophila strains bearing homozygous mutagenized autosomes (obtained from C. Zuker) for hypersensitivity to methyl methanesulfonate (MMS) and nitrogen mustard (HN2). Testing of 2585 second-chromosome lines resulted in the recovery of 18 mutants, 8 of which were alleles of known genes. The remaining 10 second-chromosome mutants were solely sensitive to MMS and define 8 new mutagen-sensitive genes (mus212-mus219). Testing of 3690 third chromosomes led to the identification of 60 third-chromosome mutants, 44 of which were alleles of known genes. The remaining 16 mutants define 14 new mutagen-sensitive genes (mus314-mus327). We have initiated efforts to identify these genes at the molecular level and report here the first two identified. The HN2-sensitive mus322 mutant defines the Drosophila ortholog of the yeast snm1 gene, and the MMS- and HN2-sensitive mus301 mutant defines the Drosophila ortholog of the human HEL308 gene. We have also identified a second-chromosome mutant, mus215ZIII-2059, that uniformly reduces the frequency of meiotic recombination to <3% of that observed in wild type and thus defines a function required for both DNA repair and meiotic recombination. At least one allele of each new gene identified in this study is available at the Bloomington Stock Center.

Original languageEnglish (US)
Pages (from-to)217-231
Number of pages15
JournalGenetics
Volume167
Issue number1
DOIs
StatePublished - May 2004

Fingerprint

Mutagens
Drosophila
Methyl Methanesulfonate
Genes
Chromosomes
Alleles
DNA Repair
Genetic Recombination
Mechlorethamine
Chromosomes, Human, Pair 10
Hypersensitivity
Yeasts

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Laurencon, A., Orme, C. M., Peters, H. K., Boulton, C. L., Vladar, E. K., Langley, S. A., ... Burtis, K. C. (2004). A large-scale screen for mutagen-sensitive loci in Drosophila. Genetics, 167(1), 217-231. https://doi.org/10.1534/genetics.167.1.217

A large-scale screen for mutagen-sensitive loci in Drosophila. / Laurencon, Anne; Orme, Charisse M.; Peters, Heather K.; Boulton, Christina L.; Vladar, Eszter K.; Langley, Sasha A.; Bakis, Emmanuel P.; Harris, David T.; Harris, Nathan J.; Wayson, Sarah M.; Hawley, R. Scott; Burtis, Kenneth C.

In: Genetics, Vol. 167, No. 1, 05.2004, p. 217-231.

Research output: Contribution to journalArticle

Laurencon, A, Orme, CM, Peters, HK, Boulton, CL, Vladar, EK, Langley, SA, Bakis, EP, Harris, DT, Harris, NJ, Wayson, SM, Hawley, RS & Burtis, KC 2004, 'A large-scale screen for mutagen-sensitive loci in Drosophila', Genetics, vol. 167, no. 1, pp. 217-231. https://doi.org/10.1534/genetics.167.1.217
Laurencon A, Orme CM, Peters HK, Boulton CL, Vladar EK, Langley SA et al. A large-scale screen for mutagen-sensitive loci in Drosophila. Genetics. 2004 May;167(1):217-231. https://doi.org/10.1534/genetics.167.1.217
Laurencon, Anne ; Orme, Charisse M. ; Peters, Heather K. ; Boulton, Christina L. ; Vladar, Eszter K. ; Langley, Sasha A. ; Bakis, Emmanuel P. ; Harris, David T. ; Harris, Nathan J. ; Wayson, Sarah M. ; Hawley, R. Scott ; Burtis, Kenneth C. / A large-scale screen for mutagen-sensitive loci in Drosophila. In: Genetics. 2004 ; Vol. 167, No. 1. pp. 217-231.
@article{cac9422f0b684b04bc88a8a235eba82e,
title = "A large-scale screen for mutagen-sensitive loci in Drosophila",
abstract = "In a screen for new DNA repair mutants, we tested 6275 Drosophila strains bearing homozygous mutagenized autosomes (obtained from C. Zuker) for hypersensitivity to methyl methanesulfonate (MMS) and nitrogen mustard (HN2). Testing of 2585 second-chromosome lines resulted in the recovery of 18 mutants, 8 of which were alleles of known genes. The remaining 10 second-chromosome mutants were solely sensitive to MMS and define 8 new mutagen-sensitive genes (mus212-mus219). Testing of 3690 third chromosomes led to the identification of 60 third-chromosome mutants, 44 of which were alleles of known genes. The remaining 16 mutants define 14 new mutagen-sensitive genes (mus314-mus327). We have initiated efforts to identify these genes at the molecular level and report here the first two identified. The HN2-sensitive mus322 mutant defines the Drosophila ortholog of the yeast snm1 gene, and the MMS- and HN2-sensitive mus301 mutant defines the Drosophila ortholog of the human HEL308 gene. We have also identified a second-chromosome mutant, mus215ZIII-2059, that uniformly reduces the frequency of meiotic recombination to <3{\%} of that observed in wild type and thus defines a function required for both DNA repair and meiotic recombination. At least one allele of each new gene identified in this study is available at the Bloomington Stock Center.",
author = "Anne Laurencon and Orme, {Charisse M.} and Peters, {Heather K.} and Boulton, {Christina L.} and Vladar, {Eszter K.} and Langley, {Sasha A.} and Bakis, {Emmanuel P.} and Harris, {David T.} and Harris, {Nathan J.} and Wayson, {Sarah M.} and Hawley, {R. Scott} and Burtis, {Kenneth C.}",
year = "2004",
month = "5",
doi = "10.1534/genetics.167.1.217",
language = "English (US)",
volume = "167",
pages = "217--231",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "1",

}

TY - JOUR

T1 - A large-scale screen for mutagen-sensitive loci in Drosophila

AU - Laurencon, Anne

AU - Orme, Charisse M.

AU - Peters, Heather K.

AU - Boulton, Christina L.

AU - Vladar, Eszter K.

AU - Langley, Sasha A.

AU - Bakis, Emmanuel P.

AU - Harris, David T.

AU - Harris, Nathan J.

AU - Wayson, Sarah M.

AU - Hawley, R. Scott

AU - Burtis, Kenneth C.

PY - 2004/5

Y1 - 2004/5

N2 - In a screen for new DNA repair mutants, we tested 6275 Drosophila strains bearing homozygous mutagenized autosomes (obtained from C. Zuker) for hypersensitivity to methyl methanesulfonate (MMS) and nitrogen mustard (HN2). Testing of 2585 second-chromosome lines resulted in the recovery of 18 mutants, 8 of which were alleles of known genes. The remaining 10 second-chromosome mutants were solely sensitive to MMS and define 8 new mutagen-sensitive genes (mus212-mus219). Testing of 3690 third chromosomes led to the identification of 60 third-chromosome mutants, 44 of which were alleles of known genes. The remaining 16 mutants define 14 new mutagen-sensitive genes (mus314-mus327). We have initiated efforts to identify these genes at the molecular level and report here the first two identified. The HN2-sensitive mus322 mutant defines the Drosophila ortholog of the yeast snm1 gene, and the MMS- and HN2-sensitive mus301 mutant defines the Drosophila ortholog of the human HEL308 gene. We have also identified a second-chromosome mutant, mus215ZIII-2059, that uniformly reduces the frequency of meiotic recombination to <3% of that observed in wild type and thus defines a function required for both DNA repair and meiotic recombination. At least one allele of each new gene identified in this study is available at the Bloomington Stock Center.

AB - In a screen for new DNA repair mutants, we tested 6275 Drosophila strains bearing homozygous mutagenized autosomes (obtained from C. Zuker) for hypersensitivity to methyl methanesulfonate (MMS) and nitrogen mustard (HN2). Testing of 2585 second-chromosome lines resulted in the recovery of 18 mutants, 8 of which were alleles of known genes. The remaining 10 second-chromosome mutants were solely sensitive to MMS and define 8 new mutagen-sensitive genes (mus212-mus219). Testing of 3690 third chromosomes led to the identification of 60 third-chromosome mutants, 44 of which were alleles of known genes. The remaining 16 mutants define 14 new mutagen-sensitive genes (mus314-mus327). We have initiated efforts to identify these genes at the molecular level and report here the first two identified. The HN2-sensitive mus322 mutant defines the Drosophila ortholog of the yeast snm1 gene, and the MMS- and HN2-sensitive mus301 mutant defines the Drosophila ortholog of the human HEL308 gene. We have also identified a second-chromosome mutant, mus215ZIII-2059, that uniformly reduces the frequency of meiotic recombination to <3% of that observed in wild type and thus defines a function required for both DNA repair and meiotic recombination. At least one allele of each new gene identified in this study is available at the Bloomington Stock Center.

UR - http://www.scopus.com/inward/record.url?scp=2942562515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942562515&partnerID=8YFLogxK

U2 - 10.1534/genetics.167.1.217

DO - 10.1534/genetics.167.1.217

M3 - Article

C2 - 15166149

AN - SCOPUS:2942562515

VL - 167

SP - 217

EP - 231

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 1

ER -