A large and phylogenetically diverse class of type 1 opsins lacking a canonical retinal binding site

Erin A. Becker, Andrew I. Yao, Phillip M. Seitzer, Tobias Kind, Ting Wang, Rich Eigenheer, Katie S Y Shao, Vladimir Yarov-Yarovoy, Marc T. Facciotti

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Opsins are photosensitive proteins catalyzing light-dependent processes across the tree of life. For both microbial (type 1) and metazoan (type 2) opsins, photosensing depends upon covalent interaction between a retinal chromophore and a conserved lysine residue. Despite recent discoveries of potential opsin homologs lacking this residue, phylogenetic dispersal and functional significance of these abnormal sequences have not yet been investigated. We report discovery of a large group of putatively non-retinal binding opsins, present in a number of fungal and microbial genomes and comprising nearly 30% of opsins in the Halobacteriacea, a model clade for opsin photobiology. We report phylogenetic analyses, structural modeling, genomic context analysis and biochemistry, to describe the evolutionary relationship of these recently described proteins with other opsins, show that they are expressed and do not bind retinal in a canonical manner. Given these data, we propose a hypothesis that these abnormal opsin homologs may represent a novel family of sensory opsins which may be involved in taxis response to one or more non-light stimuli. If true, this finding would challenge our current understanding of microbial opsins as a light-specific sensory family, and provides a potential analogy with the highly diverse signaling capabilities of the eukaryotic G-protein coupled receptors (GPCRs), of which metazoan type 2 opsins are a light-specific sub-clade.

Original languageEnglish (US)
Article numbere0156543
JournalPLoS One
Volume11
Issue number6
DOIs
StatePublished - Jun 1 2016

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Becker, E. A., Yao, A. I., Seitzer, P. M., Kind, T., Wang, T., Eigenheer, R., Shao, K. S. Y., Yarov-Yarovoy, V., & Facciotti, M. T. (2016). A large and phylogenetically diverse class of type 1 opsins lacking a canonical retinal binding site. PLoS One, 11(6), [e0156543]. https://doi.org/10.1371/journal.pone.0156543