A human lymphocyte-associated antigen involved in cell-mediated lympholysis

James Hildreth, F. M. Gotch, P. D K Hildreth, A. J. McMichael

Research output: Contribution to journalArticle

227 Citations (Scopus)

Abstract

Human lymphocyte function antigen (HLFA) is a cell surface protein defined by two monoclonal antibodies MHM23 and MHM24. It is present on both B and T lymphocytes but in greater amounts on the latter. Both antibodies precipitated antigen, from radiolabeled HSB-2 cells, which ran as two chains on sodium dodecyl sulfate polyacrylamide gel electrophoresis at 180 and 94 kDa. Neither antibody inhibited binding of the other, indicating that distinct epitopes were recognized. Both antibodies were shown to inhibit HLA-restricted lysis of influenza virus-infected and Epstein-Barr virus-transformed target cells by cytotoxic T lymphocytes. Blocking occurred at the level of the effector cells and in the presence of subsaturating concentrations of antibody. Both reagents also inhibited lysis of K562 cells, mediated by natural killer cells. These blocking effects differ from the inhibitory effects of monoclonal anti-HLA ABC and anti-suppressor cytotoxic T cell antibodies which inhibit only HLA-restricted lysis when present in saturating amounts. It is concluded therefore that HLFA is likely to be involved in the nonspecific adherence or lytic functions of killer cells rather than specific antigen recognition.

Original languageEnglish (US)
Pages (from-to)202-208
Number of pages7
JournalEuropean Journal of Immunology
Volume13
Issue number3
StatePublished - 1983
Externally publishedYes

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Lymphocytes
Antigens
Antibodies
T-Lymphocytes
K562 Cells
Cytotoxic T-Lymphocytes
Orthomyxoviridae
Human Herpesvirus 4
Sodium Dodecyl Sulfate
Natural Killer Cells
Epitopes
Polyacrylamide Gel Electrophoresis
Membrane Proteins
B-Lymphocytes
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Hildreth, J., Gotch, F. M., Hildreth, P. D. K., & McMichael, A. J. (1983). A human lymphocyte-associated antigen involved in cell-mediated lympholysis. European Journal of Immunology, 13(3), 202-208.

A human lymphocyte-associated antigen involved in cell-mediated lympholysis. / Hildreth, James; Gotch, F. M.; Hildreth, P. D K; McMichael, A. J.

In: European Journal of Immunology, Vol. 13, No. 3, 1983, p. 202-208.

Research output: Contribution to journalArticle

Hildreth, J, Gotch, FM, Hildreth, PDK & McMichael, AJ 1983, 'A human lymphocyte-associated antigen involved in cell-mediated lympholysis', European Journal of Immunology, vol. 13, no. 3, pp. 202-208.
Hildreth, James ; Gotch, F. M. ; Hildreth, P. D K ; McMichael, A. J. / A human lymphocyte-associated antigen involved in cell-mediated lympholysis. In: European Journal of Immunology. 1983 ; Vol. 13, No. 3. pp. 202-208.
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