Abstract
Effective comparative mapping inference utilizing developing gene maps of animal species requires the inclusion of anchored reference loci that are homologous to genes mapped in the more 'gene-dense' mouse and human maps. Nominated anchor loci, termed comparative anchor tagged sequences (CATS), have been ordered in the mouse linkage map, but due to the dearth of common polymorphisms among human coding genes have not been well represented in human linkage maps. We present here an ordered framework map of 314 comparative anchor markers in humans based on mapping analysis in the Genebridge 4 panel of radiation hybrid cell lines, plus empirically optimized CATS PCR primers which detect these markers. The ordering of these homologous gene markers in human and mouse maps provides a framework for comparative gene mapping of representative mammalian species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 477-484 |
| Number of pages | 8 |
| Journal | Journal of Heredity |
| Volume | 90 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 1999 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Genetics
- Genetics(clinical)
Cite this
A human genome map of comparative anchor tagged sequences. / Chen, Z. Q.; Lautenberger, J. A.; Lyons, Leslie A; McKenzie, L.; O'Brien, S. J.
In: Journal of Heredity, Vol. 90, No. 4, 07.1999, p. 477-484.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A human genome map of comparative anchor tagged sequences
AU - Chen, Z. Q.
AU - Lautenberger, J. A.
AU - Lyons, Leslie A
AU - McKenzie, L.
AU - O'Brien, S. J.
PY - 1999/7
Y1 - 1999/7
N2 - Effective comparative mapping inference utilizing developing gene maps of animal species requires the inclusion of anchored reference loci that are homologous to genes mapped in the more 'gene-dense' mouse and human maps. Nominated anchor loci, termed comparative anchor tagged sequences (CATS), have been ordered in the mouse linkage map, but due to the dearth of common polymorphisms among human coding genes have not been well represented in human linkage maps. We present here an ordered framework map of 314 comparative anchor markers in humans based on mapping analysis in the Genebridge 4 panel of radiation hybrid cell lines, plus empirically optimized CATS PCR primers which detect these markers. The ordering of these homologous gene markers in human and mouse maps provides a framework for comparative gene mapping of representative mammalian species.
AB - Effective comparative mapping inference utilizing developing gene maps of animal species requires the inclusion of anchored reference loci that are homologous to genes mapped in the more 'gene-dense' mouse and human maps. Nominated anchor loci, termed comparative anchor tagged sequences (CATS), have been ordered in the mouse linkage map, but due to the dearth of common polymorphisms among human coding genes have not been well represented in human linkage maps. We present here an ordered framework map of 314 comparative anchor markers in humans based on mapping analysis in the Genebridge 4 panel of radiation hybrid cell lines, plus empirically optimized CATS PCR primers which detect these markers. The ordering of these homologous gene markers in human and mouse maps provides a framework for comparative gene mapping of representative mammalian species.
UR - http://www.scopus.com/inward/record.url?scp=0032879329&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032879329&partnerID=8YFLogxK
U2 - 10.1093/jhered/90.4.477
DO - 10.1093/jhered/90.4.477
M3 - Article
C2 - 10485137
AN - SCOPUS:0032879329
VL - 90
SP - 477
EP - 484
JO - Journal of Heredity
JF - Journal of Heredity
SN - 0022-1503
IS - 4
ER -