Abstract
Mitochondrial toxicity is emerging as a major mechanism underlying serious human health consequences. This work performs a high-throughput screen (HTS) of 176 environmental chemicals for mitochondrial toxicity utilizing a previously reported biosensor platform. This established HTS confirmed known mitochondrial toxins and identified novel mitotochondrial uncouplers such as 2, 2′-Methylenebis(4-chlorophenol) and pentachlorophenol. It also identified a mitochondrial ‘structure activity relationship’ (SAR) in the sense that multiple environmental chlorophenols are mitochondrial inhibitors and uncouplers. This study demonstrates proof-of-concept that a mitochondrial HTS assay detects known and novel environmental mitotoxicants, and could be used to quickly evaluate human health risks from mitotoxicants in the environment.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 79-83 |
| Number of pages | 5 |
| Journal | Mitochondrion |
| Volume | 31 |
| DOIs | |
| State | Published - Nov 1 2016 |
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Keywords
- Bioenergetics
- Biosensor
- Cell-based assay
- Environmental chemicals
- High-throughput screening
- Mitochondria
- Oxygen
- Toxicants
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology
- Cell Biology
Cite this
A high-throughput screen for mitochondrial function reveals known and novel mitochondrial toxicants in a library of environmental agents. / Datta, Sandipan; Sahdeo, Sunil; Gray, Jennifer A.; Morriseau, Christophe; Hammock, Bruce D.; Cortopassi, Gino A.
In: Mitochondrion, Vol. 31, 01.11.2016, p. 79-83.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A high-throughput screen for mitochondrial function reveals known and novel mitochondrial toxicants in a library of environmental agents
AU - Datta, Sandipan
AU - Sahdeo, Sunil
AU - Gray, Jennifer A.
AU - Morriseau, Christophe
AU - Hammock, Bruce D.
AU - Cortopassi, Gino A
PY - 2016/11/1
Y1 - 2016/11/1
N2 - Mitochondrial toxicity is emerging as a major mechanism underlying serious human health consequences. This work performs a high-throughput screen (HTS) of 176 environmental chemicals for mitochondrial toxicity utilizing a previously reported biosensor platform. This established HTS confirmed known mitochondrial toxins and identified novel mitotochondrial uncouplers such as 2, 2′-Methylenebis(4-chlorophenol) and pentachlorophenol. It also identified a mitochondrial ‘structure activity relationship’ (SAR) in the sense that multiple environmental chlorophenols are mitochondrial inhibitors and uncouplers. This study demonstrates proof-of-concept that a mitochondrial HTS assay detects known and novel environmental mitotoxicants, and could be used to quickly evaluate human health risks from mitotoxicants in the environment.
AB - Mitochondrial toxicity is emerging as a major mechanism underlying serious human health consequences. This work performs a high-throughput screen (HTS) of 176 environmental chemicals for mitochondrial toxicity utilizing a previously reported biosensor platform. This established HTS confirmed known mitochondrial toxins and identified novel mitotochondrial uncouplers such as 2, 2′-Methylenebis(4-chlorophenol) and pentachlorophenol. It also identified a mitochondrial ‘structure activity relationship’ (SAR) in the sense that multiple environmental chlorophenols are mitochondrial inhibitors and uncouplers. This study demonstrates proof-of-concept that a mitochondrial HTS assay detects known and novel environmental mitotoxicants, and could be used to quickly evaluate human health risks from mitotoxicants in the environment.
KW - Bioenergetics
KW - Biosensor
KW - Cell-based assay
KW - Environmental chemicals
KW - High-throughput screening
KW - Mitochondria
KW - Oxygen
KW - Toxicants
UR - http://www.scopus.com/inward/record.url?scp=84994701916&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84994701916&partnerID=8YFLogxK
U2 - 10.1016/j.mito.2016.10.001
DO - 10.1016/j.mito.2016.10.001
M3 - Article
C2 - 27717841
AN - SCOPUS:84994701916
VL - 31
SP - 79
EP - 83
JO - Mitochondrion
JF - Mitochondrion
SN - 1567-7249
ER -