A high-throughput screen for mitochondrial function reveals known and novel mitochondrial toxicants in a library of environmental agents

Sandipan Datta, Sunil Sahdeo, Jennifer A. Gray, Christophe Morriseau, Bruce D. Hammock, Gino A Cortopassi

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Mitochondrial toxicity is emerging as a major mechanism underlying serious human health consequences. This work performs a high-throughput screen (HTS) of 176 environmental chemicals for mitochondrial toxicity utilizing a previously reported biosensor platform. This established HTS confirmed known mitochondrial toxins and identified novel mitotochondrial uncouplers such as 2, 2′-Methylenebis(4-chlorophenol) and pentachlorophenol. It also identified a mitochondrial ‘structure activity relationship’ (SAR) in the sense that multiple environmental chlorophenols are mitochondrial inhibitors and uncouplers. This study demonstrates proof-of-concept that a mitochondrial HTS assay detects known and novel environmental mitotoxicants, and could be used to quickly evaluate human health risks from mitotoxicants in the environment.

Original languageEnglish (US)
Pages (from-to)79-83
Number of pages5
JournalMitochondrion
Volume31
DOIs
StatePublished - Nov 1 2016

Keywords

  • Bioenergetics
  • Biosensor
  • Cell-based assay
  • Environmental chemicals
  • High-throughput screening
  • Mitochondria
  • Oxygen
  • Toxicants

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

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