A high-throughput screen for mitochondrial function reveals known and novel mitochondrial toxicants in a library of environmental agents

Sandipan Datta, Sunil Sahdeo, Jennifer A. Gray, Christophe Morriseau, Bruce D. Hammock, Gino A Cortopassi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mitochondrial toxicity is emerging as a major mechanism underlying serious human health consequences. This work performs a high-throughput screen (HTS) of 176 environmental chemicals for mitochondrial toxicity utilizing a previously reported biosensor platform. This established HTS confirmed known mitochondrial toxins and identified novel mitotochondrial uncouplers such as 2, 2′-Methylenebis(4-chlorophenol) and pentachlorophenol. It also identified a mitochondrial ‘structure activity relationship’ (SAR) in the sense that multiple environmental chlorophenols are mitochondrial inhibitors and uncouplers. This study demonstrates proof-of-concept that a mitochondrial HTS assay detects known and novel environmental mitotoxicants, and could be used to quickly evaluate human health risks from mitotoxicants in the environment.

Original languageEnglish (US)
Pages (from-to)79-83
Number of pages5
JournalMitochondrion
Volume31
DOIs
StatePublished - Nov 1 2016

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Libraries
Chlorophenols
Pentachlorophenol
Health
Biosensing Techniques
Structure-Activity Relationship
4-chlorophenol

Keywords

  • Bioenergetics
  • Biosensor
  • Cell-based assay
  • Environmental chemicals
  • High-throughput screening
  • Mitochondria
  • Oxygen
  • Toxicants

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

Cite this

A high-throughput screen for mitochondrial function reveals known and novel mitochondrial toxicants in a library of environmental agents. / Datta, Sandipan; Sahdeo, Sunil; Gray, Jennifer A.; Morriseau, Christophe; Hammock, Bruce D.; Cortopassi, Gino A.

In: Mitochondrion, Vol. 31, 01.11.2016, p. 79-83.

Research output: Contribution to journalArticle

Datta, Sandipan ; Sahdeo, Sunil ; Gray, Jennifer A. ; Morriseau, Christophe ; Hammock, Bruce D. ; Cortopassi, Gino A. / A high-throughput screen for mitochondrial function reveals known and novel mitochondrial toxicants in a library of environmental agents. In: Mitochondrion. 2016 ; Vol. 31. pp. 79-83.
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