Abstract
Mitochondrial toxicity is emerging as a major mechanism underlying serious human health consequences. This work performs a high-throughput screen (HTS) of 176 environmental chemicals for mitochondrial toxicity utilizing a previously reported biosensor platform. This established HTS confirmed known mitochondrial toxins and identified novel mitotochondrial uncouplers such as 2, 2′-Methylenebis(4-chlorophenol) and pentachlorophenol. It also identified a mitochondrial ‘structure activity relationship’ (SAR) in the sense that multiple environmental chlorophenols are mitochondrial inhibitors and uncouplers. This study demonstrates proof-of-concept that a mitochondrial HTS assay detects known and novel environmental mitotoxicants, and could be used to quickly evaluate human health risks from mitotoxicants in the environment.
Original language | English (US) |
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Pages (from-to) | 79-83 |
Number of pages | 5 |
Journal | Mitochondrion |
Volume | 31 |
DOIs | |
State | Published - Nov 1 2016 |
Keywords
- Bioenergetics
- Biosensor
- Cell-based assay
- Environmental chemicals
- High-throughput screening
- Mitochondria
- Oxygen
- Toxicants
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology
- Cell Biology