A high throughput microfluidic device for generating multiple human microtissues with perfused capillaries

Y. H. Hsu, M. L. Moya, C. C.W. Hughes, Steven George, A. P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

This paper reports a model system that can generate multiple human microtissues with perfused human capillaries on a single microfluidic platform. Inspired by the understanding of vasculogenesis process, a high throughput microfluidic platform that can provide a three-dimensional biologically and anatomically correct environment was developed. A physiological environment with controlled interstitial flow was generated in each cell construct, seeded with stromal cells and endothelial cells, and the vasculogenic process was initiated to develop vascular network. Perfused capillaries in developed human microtissues were evidenced within 18-day culture by flowing 1 μm fluorescent polystyrene beads and confocal imaging.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1382-1384
Number of pages3
Volume2
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Interstitial flow
  • Microtissue
  • Perfused capillaries
  • Vasculogenesis

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Hsu, Y. H., Moya, M. L., Hughes, C. C. W., George, S., & Lee, A. P. (2011). A high throughput microfluidic device for generating multiple human microtissues with perfused capillaries. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 2, pp. 1382-1384)