A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities

Prabhakara V Choudary; Xiang Yang Liu; Prabhakara V. Choudary

doi:10.1007/BF02787981

A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities

Prabhakara V Choudary, Xiang Yang Liu, Prabhakara V. Choudary

Psychiatry and Behavioral Sciences

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The authors describe a microplate-based high-throughput procedure for rapid assay of the enzyme activities of nitrate reductase and nitrite reductase, using extremely small volumes of reagents. The new procedure offers the advantages of rapidity, small sample size-nanoliter volumes, low cost, and a dramatic increase in the throughput sample number that can be analyzed simultaneously. Additional advantages can be accessed by using microplate reader application software packages that permit assigning a group type to the wells, recording of the data on exportable data files and exercising the option of using the kinetic or endpoint reading modes. The assay can also be used independently for detecting nitrite residues/contamination in environmental/food samples.

Original language	English (US)
Pages (from-to)	29-36
Number of pages	8
Journal	Applied Biochemistry and Biotechnology
Volume	62
Issue number	1
DOIs	https://doi.org/10.1007/BF02787981
State	Published - Jan 1997

Keywords

assimilation
Candida utilis
environment
enzyme assay
food yeast, microplate
nitrate
nitric oxide synthase
nitrite
water

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Environmental Engineering
Bioengineering
Molecular Biology
Biotechnology
Biochemistry
Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1007/BF02787981

Fingerprint Dive into the research topics of 'A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities'. Together they form a unique fingerprint.

Cite this

@article{b34377d32bc249dcb9a1dca1efb918d8,

title = "A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities",

abstract = "The authors describe a microplate-based high-throughput procedure for rapid assay of the enzyme activities of nitrate reductase and nitrite reductase, using extremely small volumes of reagents. The new procedure offers the advantages of rapidity, small sample size-nanoliter volumes, low cost, and a dramatic increase in the throughput sample number that can be analyzed simultaneously. Additional advantages can be accessed by using microplate reader application software packages that permit assigning a group type to the wells, recording of the data on exportable data files and exercising the option of using the kinetic or endpoint reading modes. The assay can also be used independently for detecting nitrite residues/contamination in environmental/food samples.",

keywords = "assimilation, Candida utilis, environment, enzyme assay, food yeast, microplate, nitrate, nitric oxide synthase, nitrite, water",

author = "Choudary, {Prabhakara V} and Liu, {Xiang Yang} and Choudary, {Prabhakara V.}",

year = "1997",

month = jan,

doi = "10.1007/BF02787981",

language = "English (US)",

volume = "62",

pages = "29--36",

journal = "Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology",

issn = "0273-2289",

publisher = "Humana Press",

number = "1",

}

TY - JOUR

T1 - A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities

AU - Choudary, Prabhakara V

AU - Liu, Xiang Yang

AU - Choudary, Prabhakara V.

PY - 1997/1

Y1 - 1997/1

N2 - The authors describe a microplate-based high-throughput procedure for rapid assay of the enzyme activities of nitrate reductase and nitrite reductase, using extremely small volumes of reagents. The new procedure offers the advantages of rapidity, small sample size-nanoliter volumes, low cost, and a dramatic increase in the throughput sample number that can be analyzed simultaneously. Additional advantages can be accessed by using microplate reader application software packages that permit assigning a group type to the wells, recording of the data on exportable data files and exercising the option of using the kinetic or endpoint reading modes. The assay can also be used independently for detecting nitrite residues/contamination in environmental/food samples.

AB - The authors describe a microplate-based high-throughput procedure for rapid assay of the enzyme activities of nitrate reductase and nitrite reductase, using extremely small volumes of reagents. The new procedure offers the advantages of rapidity, small sample size-nanoliter volumes, low cost, and a dramatic increase in the throughput sample number that can be analyzed simultaneously. Additional advantages can be accessed by using microplate reader application software packages that permit assigning a group type to the wells, recording of the data on exportable data files and exercising the option of using the kinetic or endpoint reading modes. The assay can also be used independently for detecting nitrite residues/contamination in environmental/food samples.

KW - assimilation

KW - Candida utilis

KW - environment

KW - enzyme assay

KW - food yeast, microplate

KW - nitrate

KW - nitric oxide synthase

KW - nitrite

KW - water

UR - http://www.scopus.com/inward/record.url?scp=0030623659&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030623659&partnerID=8YFLogxK

U2 - 10.1007/BF02787981

DO - 10.1007/BF02787981

M3 - Article

C2 - 9103606

AN - SCOPUS:0030623659

VL - 62

SP - 29

EP - 36

JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

SN - 0273-2289

IS - 1

ER -