Abstract
The authors describe a microplate-based high-throughput procedure for rapid assay of the enzyme activities of nitrate reductase and nitrite reductase, using extremely small volumes of reagents. The new procedure offers the advantages of rapidity, small sample size-nanoliter volumes, low cost, and a dramatic increase in the throughput sample number that can be analyzed simultaneously. Additional advantages can be accessed by using microplate reader application software packages that permit assigning a group type to the wells, recording of the data on exportable data files and exercising the option of using the kinetic or endpoint reading modes. The assay can also be used independently for detecting nitrite residues/contamination in environmental/food samples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 29-36 |
| Number of pages | 8 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume | 62 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1997 |
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Keywords
- assimilation
- Candida utilis
- environment
- enzyme assay
- food yeast, microplate
- nitrate
- nitric oxide synthase
- nitrite
- water
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Environmental Engineering
- Bioengineering
- Molecular Biology
- Biotechnology
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities. / Choudary, Prabhakara V; Liu, Xiang Yang; Choudary, Prabhakara V.
In: Applied Biochemistry and Biotechnology, Vol. 62, No. 1, 01.1997, p. 29-36.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities
AU - Choudary, Prabhakara V
AU - Liu, Xiang Yang
AU - Choudary, Prabhakara V.
PY - 1997/1
Y1 - 1997/1
N2 - The authors describe a microplate-based high-throughput procedure for rapid assay of the enzyme activities of nitrate reductase and nitrite reductase, using extremely small volumes of reagents. The new procedure offers the advantages of rapidity, small sample size-nanoliter volumes, low cost, and a dramatic increase in the throughput sample number that can be analyzed simultaneously. Additional advantages can be accessed by using microplate reader application software packages that permit assigning a group type to the wells, recording of the data on exportable data files and exercising the option of using the kinetic or endpoint reading modes. The assay can also be used independently for detecting nitrite residues/contamination in environmental/food samples.
AB - The authors describe a microplate-based high-throughput procedure for rapid assay of the enzyme activities of nitrate reductase and nitrite reductase, using extremely small volumes of reagents. The new procedure offers the advantages of rapidity, small sample size-nanoliter volumes, low cost, and a dramatic increase in the throughput sample number that can be analyzed simultaneously. Additional advantages can be accessed by using microplate reader application software packages that permit assigning a group type to the wells, recording of the data on exportable data files and exercising the option of using the kinetic or endpoint reading modes. The assay can also be used independently for detecting nitrite residues/contamination in environmental/food samples.
KW - assimilation
KW - Candida utilis
KW - environment
KW - enzyme assay
KW - food yeast, microplate
KW - nitrate
KW - nitric oxide synthase
KW - nitrite
KW - water
UR - http://www.scopus.com/inward/record.url?scp=0030623659&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030623659&partnerID=8YFLogxK
U2 - 10.1007/BF02787981
DO - 10.1007/BF02787981
M3 - Article
C2 - 9103606
AN - SCOPUS:0030623659
VL - 62
SP - 29
EP - 36
JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
SN - 0273-2289
IS - 1
ER -