A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities

Prabhakara V Choudary, Xiang Yang Liu, Prabhakara V. Choudary

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The authors describe a microplate-based high-throughput procedure for rapid assay of the enzyme activities of nitrate reductase and nitrite reductase, using extremely small volumes of reagents. The new procedure offers the advantages of rapidity, small sample size-nanoliter volumes, low cost, and a dramatic increase in the throughput sample number that can be analyzed simultaneously. Additional advantages can be accessed by using microplate reader application software packages that permit assigning a group type to the wells, recording of the data on exportable data files and exercising the option of using the kinetic or endpoint reading modes. The assay can also be used independently for detecting nitrite residues/contamination in environmental/food samples.

Original languageEnglish (US)
Pages (from-to)29-36
Number of pages8
JournalApplied Biochemistry and Biotechnology
Volume62
Issue number1
DOIs
StatePublished - Jan 1997

Fingerprint

Nitrite Reductases
Nitrate Reductase
Enzyme activity
Nitrates
Assays
Throughput
Information Storage and Retrieval
Enzyme Assays
Enzymes
Nitrites
Application programs
Software packages
Sample Size
Reading
Contamination
Software
Costs and Cost Analysis
Food
Kinetics
Costs

Keywords

  • assimilation
  • Candida utilis
  • environment
  • enzyme assay
  • food yeast, microplate
  • nitrate
  • nitric oxide synthase
  • nitrite
  • water

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Molecular Biology
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities. / Choudary, Prabhakara V; Liu, Xiang Yang; Choudary, Prabhakara V.

In: Applied Biochemistry and Biotechnology, Vol. 62, No. 1, 01.1997, p. 29-36.

Research output: Contribution to journalArticle

Choudary, Prabhakara V ; Liu, Xiang Yang ; Choudary, Prabhakara V. / A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities. In: Applied Biochemistry and Biotechnology. 1997 ; Vol. 62, No. 1. pp. 29-36.
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