A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities

Prabhakara V Choudary; Xiang Yang Liu; Prabhakara V. Choudary

doi:10.1007/BF02787981

A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities

Prabhakara V Choudary, Xiang Yang Liu, Prabhakara V. Choudary

Psychiatry and Behavioral Sciences

Research output: Contribution to journal › Article

1 Scopus citations

Abstract

The authors describe a microplate-based high-throughput procedure for rapid assay of the enzyme activities of nitrate reductase and nitrite reductase, using extremely small volumes of reagents. The new procedure offers the advantages of rapidity, small sample size-nanoliter volumes, low cost, and a dramatic increase in the throughput sample number that can be analyzed simultaneously. Additional advantages can be accessed by using microplate reader application software packages that permit assigning a group type to the wells, recording of the data on exportable data files and exercising the option of using the kinetic or endpoint reading modes. The assay can also be used independently for detecting nitrite residues/contamination in environmental/food samples.

Original language	English (US)
Pages (from-to)	29-36
Number of pages	8
Journal	Applied Biochemistry and Biotechnology
Volume	62
Issue number	1
DOIs	https://doi.org/10.1007/BF02787981
State	Published - Jan 1997

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Keywords

assimilation
Candida utilis
environment
enzyme assay
food yeast, microplate
nitrate
nitric oxide synthase
nitrite
water

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Environmental Engineering
Bioengineering
Molecular Biology
Biotechnology
Biochemistry
Biochemistry, Genetics and Molecular Biology(all)

Cite this

Choudary, P. V., Liu, X. Y., & Choudary, P. V. (1997). A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities. Applied Biochemistry and Biotechnology, 62(1), 29-36. https://doi.org/10.1007/BF02787981

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10.1007/BF02787981