A high sensitivity multi-spectral three-dimensional fluorescence optical tomography system for small animal imaging

Changqing Li, Gregory Mitchell, Joyita Dutta, Sangtae Ahn, Richard M. Leahy, Simon R Cherry

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have designed a three dimensional (3D) fluorescence optical tomography system for small animal imaging based on an innovative system geometry that uses a truncated conical mirror which permits the entire surface of the animal to be viewed simultaneously by a single CCD camera. Compared with traditional approaches that employ a flat mirror, the conical mirror system has approximately 3 times better measurement sensitivity. By utilizing a fast switching filter wheel (switching time < 100 milliseconds), emission data at multiple wavelengths can be efficiently collected. An array of appropriately shaped neutral density filters, mounted on a linear stage, can be used to increase the system measurement dynamic range by 3 orders of magnitude. An x-y galvo mirror scanning system makes it possible to scan a collimated laser beam to any location on the mouse surface. A pattern of structured light incident on the animal surface is used to extract the surface geometry. A finite element based algorithm is applied to model photon propagation in the turbid media and a preconditioned conjugate gradient (PCG) method is used to solve the large linear system matrix. The reconstruction algorithm and the system performance are evaluated by phantom experiments.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7174
DOIs
StatePublished - 2009
EventOptical Tomography and Spectroscopy of Tissue VIII - San Jose, CA, United States
Duration: Jan 25 2009Jan 27 2009

Other

OtherOptical Tomography and Spectroscopy of Tissue VIII
CountryUnited States
CitySan Jose, CA
Period1/25/091/27/09

Keywords

  • Conical mirror
  • Fluorescence optical tomography
  • Small animal imaging

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Li, C., Mitchell, G., Dutta, J., Ahn, S., Leahy, R. M., & Cherry, S. R. (2009). A high sensitivity multi-spectral three-dimensional fluorescence optical tomography system for small animal imaging. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7174). [717415] https://doi.org/10.1117/12.809705