A high-resolution radiation hybrid map of rhesus macaque chromosome 5 identifies rearrangements in the genome assembly

Genesio M. Karere; Lutz Froenicke; Lee Millon; James E. Womack; Leslie A Lyons

doi:10.1016/j.ygeno.2008.05.013

A high-resolution radiation hybrid map of rhesus macaque chromosome 5 identifies rearrangements in the genome assembly

Genesio M. Karere, Lutz Froenicke, Lee Millon, James E. Womack, Leslie A Lyons

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A 10,000-rad radiation hybrid (RH) cell panel of the rhesus macaque was generated to construct a comprehensive RH map of chromosome 5. The map represents 218 markers typed in 185 RH clones. The 4846-cR map has an average marker spacing of 798 kb. Alignments of the RH map to macaque and human genome sequences confirm a large inversion and reveal a previously unreported telomeric inversion. The macaque genome sequence indicates small translocations from the ancestral homolog of macaque chromosome 5 to macaque chromosomes 1 and 6. The RH map suggests that these are probably assembly artifacts. Unlike the genome sequence, the RH mapping data indicate the conservation of synteny between macaque chromosome 5 and human chromosome 4. This study shows that the 10,000-rad panel is appropriate for the generation of a high-resolution whole-genome RH map suitable for the verification of the rhesus genome assembly.

Original language	English (US)
Pages (from-to)	210-218
Number of pages	9
Journal	Genomics
Volume	92
Issue number	4
DOIs	https://doi.org/10.1016/j.ygeno.2008.05.013
State	Published - Oct 2008
Externally published	Yes

Keywords

Comparative mapping
Macaque and human genomes
Radiation hybrid
RH map
Rhesus macaque

ASJC Scopus subject areas

Genetics

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10.1016/j.ygeno.2008.05.013

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title = "A high-resolution radiation hybrid map of rhesus macaque chromosome 5 identifies rearrangements in the genome assembly",

abstract = "A 10,000-rad radiation hybrid (RH) cell panel of the rhesus macaque was generated to construct a comprehensive RH map of chromosome 5. The map represents 218 markers typed in 185 RH clones. The 4846-cR map has an average marker spacing of 798 kb. Alignments of the RH map to macaque and human genome sequences confirm a large inversion and reveal a previously unreported telomeric inversion. The macaque genome sequence indicates small translocations from the ancestral homolog of macaque chromosome 5 to macaque chromosomes 1 and 6. The RH map suggests that these are probably assembly artifacts. Unlike the genome sequence, the RH mapping data indicate the conservation of synteny between macaque chromosome 5 and human chromosome 4. This study shows that the 10,000-rad panel is appropriate for the generation of a high-resolution whole-genome RH map suitable for the verification of the rhesus genome assembly.",

keywords = "Comparative mapping, Macaque and human genomes, Radiation hybrid, RH map, Rhesus macaque",

author = "Karere, {Genesio M.} and Lutz Froenicke and Lee Millon and Womack, {James E.} and Lyons, {Leslie A}",

year = "2008",

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T1 - A high-resolution radiation hybrid map of rhesus macaque chromosome 5 identifies rearrangements in the genome assembly

AU - Karere, Genesio M.

AU - Froenicke, Lutz

AU - Millon, Lee

AU - Womack, James E.

AU - Lyons, Leslie A

PY - 2008/10

Y1 - 2008/10

N2 - A 10,000-rad radiation hybrid (RH) cell panel of the rhesus macaque was generated to construct a comprehensive RH map of chromosome 5. The map represents 218 markers typed in 185 RH clones. The 4846-cR map has an average marker spacing of 798 kb. Alignments of the RH map to macaque and human genome sequences confirm a large inversion and reveal a previously unreported telomeric inversion. The macaque genome sequence indicates small translocations from the ancestral homolog of macaque chromosome 5 to macaque chromosomes 1 and 6. The RH map suggests that these are probably assembly artifacts. Unlike the genome sequence, the RH mapping data indicate the conservation of synteny between macaque chromosome 5 and human chromosome 4. This study shows that the 10,000-rad panel is appropriate for the generation of a high-resolution whole-genome RH map suitable for the verification of the rhesus genome assembly.

AB - A 10,000-rad radiation hybrid (RH) cell panel of the rhesus macaque was generated to construct a comprehensive RH map of chromosome 5. The map represents 218 markers typed in 185 RH clones. The 4846-cR map has an average marker spacing of 798 kb. Alignments of the RH map to macaque and human genome sequences confirm a large inversion and reveal a previously unreported telomeric inversion. The macaque genome sequence indicates small translocations from the ancestral homolog of macaque chromosome 5 to macaque chromosomes 1 and 6. The RH map suggests that these are probably assembly artifacts. Unlike the genome sequence, the RH mapping data indicate the conservation of synteny between macaque chromosome 5 and human chromosome 4. This study shows that the 10,000-rad panel is appropriate for the generation of a high-resolution whole-genome RH map suitable for the verification of the rhesus genome assembly.

KW - Comparative mapping

KW - Macaque and human genomes

KW - Radiation hybrid

KW - RH map

KW - Rhesus macaque

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