A high-resolution radiation hybrid map of rhesus macaque chromosome 5 identifies rearrangements in the genome assembly

Genesio M. Karere, Lutz Froenicke, Lee Millon, James E. Womack, Leslie A Lyons

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

A 10,000-rad radiation hybrid (RH) cell panel of the rhesus macaque was generated to construct a comprehensive RH map of chromosome 5. The map represents 218 markers typed in 185 RH clones. The 4846-cR map has an average marker spacing of 798 kb. Alignments of the RH map to macaque and human genome sequences confirm a large inversion and reveal a previously unreported telomeric inversion. The macaque genome sequence indicates small translocations from the ancestral homolog of macaque chromosome 5 to macaque chromosomes 1 and 6. The RH map suggests that these are probably assembly artifacts. Unlike the genome sequence, the RH mapping data indicate the conservation of synteny between macaque chromosome 5 and human chromosome 4. This study shows that the 10,000-rad panel is appropriate for the generation of a high-resolution whole-genome RH map suitable for the verification of the rhesus genome assembly.

Original languageEnglish (US)
Pages (from-to)210-218
Number of pages9
JournalGenomics
Volume92
Issue number4
DOIs
StatePublished - Oct 2008
Externally publishedYes

Keywords

  • Comparative mapping
  • Macaque and human genomes
  • Radiation hybrid
  • RH map
  • Rhesus macaque

ASJC Scopus subject areas

  • Genetics

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