Abstract
A 10,000-rad radiation hybrid (RH) cell panel of the rhesus macaque was generated to construct a comprehensive RH map of chromosome 5. The map represents 218 markers typed in 185 RH clones. The 4846-cR map has an average marker spacing of 798 kb. Alignments of the RH map to macaque and human genome sequences confirm a large inversion and reveal a previously unreported telomeric inversion. The macaque genome sequence indicates small translocations from the ancestral homolog of macaque chromosome 5 to macaque chromosomes 1 and 6. The RH map suggests that these are probably assembly artifacts. Unlike the genome sequence, the RH mapping data indicate the conservation of synteny between macaque chromosome 5 and human chromosome 4. This study shows that the 10,000-rad panel is appropriate for the generation of a high-resolution whole-genome RH map suitable for the verification of the rhesus genome assembly.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 210-218 |
| Number of pages | 9 |
| Journal | Genomics |
| Volume | 92 |
| Issue number | 4 |
| DOIs | |
| State | Published - Oct 2008 |
| Externally published | Yes |
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Keywords
- Comparative mapping
- Macaque and human genomes
- Radiation hybrid
- RH map
- Rhesus macaque
ASJC Scopus subject areas
- Genetics
Cite this
A high-resolution radiation hybrid map of rhesus macaque chromosome 5 identifies rearrangements in the genome assembly. / Karere, Genesio M.; Froenicke, Lutz; Millon, Lee; Womack, James E.; Lyons, Leslie A.
In: Genomics, Vol. 92, No. 4, 10.2008, p. 210-218.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A high-resolution radiation hybrid map of rhesus macaque chromosome 5 identifies rearrangements in the genome assembly
AU - Karere, Genesio M.
AU - Froenicke, Lutz
AU - Millon, Lee
AU - Womack, James E.
AU - Lyons, Leslie A
PY - 2008/10
Y1 - 2008/10
N2 - A 10,000-rad radiation hybrid (RH) cell panel of the rhesus macaque was generated to construct a comprehensive RH map of chromosome 5. The map represents 218 markers typed in 185 RH clones. The 4846-cR map has an average marker spacing of 798 kb. Alignments of the RH map to macaque and human genome sequences confirm a large inversion and reveal a previously unreported telomeric inversion. The macaque genome sequence indicates small translocations from the ancestral homolog of macaque chromosome 5 to macaque chromosomes 1 and 6. The RH map suggests that these are probably assembly artifacts. Unlike the genome sequence, the RH mapping data indicate the conservation of synteny between macaque chromosome 5 and human chromosome 4. This study shows that the 10,000-rad panel is appropriate for the generation of a high-resolution whole-genome RH map suitable for the verification of the rhesus genome assembly.
AB - A 10,000-rad radiation hybrid (RH) cell panel of the rhesus macaque was generated to construct a comprehensive RH map of chromosome 5. The map represents 218 markers typed in 185 RH clones. The 4846-cR map has an average marker spacing of 798 kb. Alignments of the RH map to macaque and human genome sequences confirm a large inversion and reveal a previously unreported telomeric inversion. The macaque genome sequence indicates small translocations from the ancestral homolog of macaque chromosome 5 to macaque chromosomes 1 and 6. The RH map suggests that these are probably assembly artifacts. Unlike the genome sequence, the RH mapping data indicate the conservation of synteny between macaque chromosome 5 and human chromosome 4. This study shows that the 10,000-rad panel is appropriate for the generation of a high-resolution whole-genome RH map suitable for the verification of the rhesus genome assembly.
KW - Comparative mapping
KW - Macaque and human genomes
KW - Radiation hybrid
KW - RH map
KW - Rhesus macaque
UR - http://www.scopus.com/inward/record.url?scp=51649093982&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=51649093982&partnerID=8YFLogxK
U2 - 10.1016/j.ygeno.2008.05.013
DO - 10.1016/j.ygeno.2008.05.013
M3 - Article
C2 - 18601997
AN - SCOPUS:51649093982
VL - 92
SP - 210
EP - 218
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 4
ER -