A high-resolution 15,000 Rad radiation hybrid panel for the domestic cat

L. H. Bach, B. Gandolfi, J. C. Grahn, L. V. Millon, Michael S Kent, K. Narfstrom, S. A. Cole, J. C. Mullikin, Robert A Grahn, Leslie A Lyons

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The current genetic and recombination maps of the cat have fewer than 3,000 markers and a resolution limit greater than 1 Mb. To complement the first-generation domestic cat maps, support higher resolution mapping studies, and aid genome assembly in specific areas as well as in the whole genome, a 15,000 Rad radiation hybrid (RH) panel for the domestic cat was generated. Fibroblasts from the female Abyssinian cat that was used to generate the cat genomic sequence were fused to a Chinese hamster cell line (A23), producing 150 hybrid lines. The clones were initially characterized using 39 short tandem repeats (STRs) and 1,536 SNP markers. The utility of whole-genome amplification in preserving and extending RH panel DNA was also tested using 10 STR markers; no significant difference in retention was observed. The resolution of the 15,000 Rad RH panel was established by constructing framework maps across 10 different 1-Mb regions on different feline chromosomes. In these regions, 2-point analysis was used to estimate RH distances, which compared favorably with the estimation of physical distances. The study demonstrates that the 15,000 Rad RH panel constitutes a powerful tool for constructing high-resolution maps, having an average resolution of 40.1 kb per marker across the ten 1-Mb regions. In addition, the RH panel will complement existing genomic resources for the domestic cat, aid in the accurate re-assemblies of the forthcoming cat genomic sequence, and support cross-species genomic comparisons.

Original languageEnglish (US)
Pages (from-to)7-14
Number of pages8
JournalCytogenetic and Genome Research
Issue number1
StatePublished - Aug 2012


  • Felis catus
  • Genetic mapping
  • Whole-genome amplification

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology


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