A high-resolution 15,000 Rad radiation hybrid panel for the domestic cat

L. H. Bach, B. Gandolfi, J. C. Grahn, L. V. Millon, Michael S Kent, K. Narfstrom, S. A. Cole, J. C. Mullikin, Robert A Grahn, Leslie A Lyons

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The current genetic and recombination maps of the cat have fewer than 3,000 markers and a resolution limit greater than 1 Mb. To complement the first-generation domestic cat maps, support higher resolution mapping studies, and aid genome assembly in specific areas as well as in the whole genome, a 15,000 Rad radiation hybrid (RH) panel for the domestic cat was generated. Fibroblasts from the female Abyssinian cat that was used to generate the cat genomic sequence were fused to a Chinese hamster cell line (A23), producing 150 hybrid lines. The clones were initially characterized using 39 short tandem repeats (STRs) and 1,536 SNP markers. The utility of whole-genome amplification in preserving and extending RH panel DNA was also tested using 10 STR markers; no significant difference in retention was observed. The resolution of the 15,000 Rad RH panel was established by constructing framework maps across 10 different 1-Mb regions on different feline chromosomes. In these regions, 2-point analysis was used to estimate RH distances, which compared favorably with the estimation of physical distances. The study demonstrates that the 15,000 Rad RH panel constitutes a powerful tool for constructing high-resolution maps, having an average resolution of 40.1 kb per marker across the ten 1-Mb regions. In addition, the RH panel will complement existing genomic resources for the domestic cat, aid in the accurate re-assemblies of the forthcoming cat genomic sequence, and support cross-species genomic comparisons.

Original languageEnglish (US)
Pages (from-to)7-14
Number of pages8
JournalCytogenetic and Genome Research
Volume137
Issue number1
DOIs
StatePublished - Aug 2012

Fingerprint

Radiation Hybrid Mapping
Cats
Genome
Microsatellite Repeats
Felidae
Cricetulus
Genetic Recombination
Single Nucleotide Polymorphism
Clone Cells
Fibroblasts
Chromosomes
Cell Line
DNA

Keywords

  • Felis catus
  • Genetic mapping
  • Whole-genome amplification

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

A high-resolution 15,000 Rad radiation hybrid panel for the domestic cat. / Bach, L. H.; Gandolfi, B.; Grahn, J. C.; Millon, L. V.; Kent, Michael S; Narfstrom, K.; Cole, S. A.; Mullikin, J. C.; Grahn, Robert A; Lyons, Leslie A.

In: Cytogenetic and Genome Research, Vol. 137, No. 1, 08.2012, p. 7-14.

Research output: Contribution to journalArticle

Bach, LH, Gandolfi, B, Grahn, JC, Millon, LV, Kent, MS, Narfstrom, K, Cole, SA, Mullikin, JC, Grahn, RA & Lyons, LA 2012, 'A high-resolution 15,000 Rad radiation hybrid panel for the domestic cat', Cytogenetic and Genome Research, vol. 137, no. 1, pp. 7-14. https://doi.org/10.1159/000339416
Bach, L. H. ; Gandolfi, B. ; Grahn, J. C. ; Millon, L. V. ; Kent, Michael S ; Narfstrom, K. ; Cole, S. A. ; Mullikin, J. C. ; Grahn, Robert A ; Lyons, Leslie A. / A high-resolution 15,000 Rad radiation hybrid panel for the domestic cat. In: Cytogenetic and Genome Research. 2012 ; Vol. 137, No. 1. pp. 7-14.
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