A heat-shock-like response with cytoskeletal disruption occurs following hydrostatic pressure in MG-63 osteosarcoma cells.

C. L. Haskin, K. A. Athanasiou, R. Klebe, I. L. Cameron

Research output: Contribution to journalArticle

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Abstract

Human osteosarcoma cells, MG-63, were exposed to a hydrostatic pressure shock of 4.0 MPa for 20 min. Changes in subcellular distribution of the cytoskeletal elements and heat shock protein 70 (hsp70) were followed by indirect immunofluorescence and by avidin-biotin-peroxidase protocols. During recovery, total cellular RNA was determined and actin and aldolase mRNA content was followed using reverse transcription-polymerase chain reaction techniques. Hydrostatic pressure caused cell rounding (but not cell death), disruption of microtubules, collapse of intermediate filaments to a perinuclear location, collapse of actin stress fibers into globular aggregates in the cytoplasm, and the formation of several large elongated intranuclear actin inclusions. During recovery, the cells flattened, reorganized microtubules, and redistributed intermediate filaments prior to the reappearance of actin stress fibers. At 20 and 60 min following the initiation of hydrostatic pressure, there was increased anti-hsp 70 staining at the nuclear membrane and concentration of hsp70 in four to six granules in the nucleus. At 120 min following the hydrostatic pressure, hsp70 showed intense staining in the cytoplasm and hsp70-containing granules in the nucleus disappeared. Cellular RNA decreased during the first 120-min posthydrostatic pressure shock and then recovered to near prehydrostatic pressure treatment levels by 240 min. Actin mRNA abundance, in relation to aldolase mRNA abundance, showed the same temporal pattern of initial decrease, followed by increase as did total RNA. Review of the literature indicated that eukaryotic cells respond to heat shock and to hydrostatic pressure by disruption of the cytoskeletal elements and by similar modifications in genetic expression. In this study, the observed responses of MG-63 cells to a 4-MPa hydrostatic pressure shock was like the reported response of mammalian cells to a 43 degrees C heat shock.

Original languageEnglish (US)
Pages (from-to)361-371
Number of pages11
JournalBiochemistry and cell biology = Biochimie et biologie cellulaire
Volume71
Issue number7-8
StatePublished - Jul 1993
Externally publishedYes

Fingerprint

Heat-Shock Response
Hydrostatic Pressure
Osteosarcoma
Hydrostatic pressure
HSP70 Heat-Shock Proteins
Actins
Shock
Stress Fibers
Fructose-Bisphosphate Aldolase
Intermediate Filaments
RNA
Microtubules
Messenger RNA
Cytoplasm
Hot Temperature
Staining and Labeling
Pressure
Intranuclear Inclusion Bodies
Recovery
Avidin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

A heat-shock-like response with cytoskeletal disruption occurs following hydrostatic pressure in MG-63 osteosarcoma cells. / Haskin, C. L.; Athanasiou, K. A.; Klebe, R.; Cameron, I. L.

In: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 71, No. 7-8, 07.1993, p. 361-371.

Research output: Contribution to journalArticle

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