A glutaminase (Gls) gene maps to mouse chromosome 1, rat chromosome 9, and human chromosome 2

Beverly Mock, Christine Kozak, Michael F Seldin, Naomi Ruff, Lawrence D'Hoostelaere, Claude Szpirer, Hector Seuanez, Steve O'Brien, Carl Banner

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

A rat cDNA clone encoding a portion of phosphate-activated glutaminase was used to identify DNA restriction fragment length polymorphisms (RFLPs) in sets of somatic cell hybrids and between wild-derived and inbred strains of mice. Segregation of rat and mouse chromosomes among somatic cell hybrids indicated assignment to rat chromosome 9 and mouse chromosome 1. Analysis of chromosome 1 alleles for several genes in an interspecific cross between Mus spretus and C3H HeJ- gld gld mice indicates that glutaminase can be positioned within 5.5 ± 2.0 cM proximal to Ctla-4. Similarly, human-hamster somatic cell hybrids were examined for RFLPs, and four human EcoRI restriction fragments were found to hybridize with the rat glutaminase probe. Two of these restriction fragments cosegregated and mapped to human chromosome 2 in a region that is syntenic with mouse chromosome 1 and rat chromosome 9.

Original languageEnglish (US)
Pages (from-to)291-297
Number of pages7
JournalGenomics
Volume5
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Mock, B., Kozak, C., Seldin, M. F., Ruff, N., D'Hoostelaere, L., Szpirer, C., Seuanez, H., O'Brien, S., & Banner, C. (1989). A glutaminase (Gls) gene maps to mouse chromosome 1, rat chromosome 9, and human chromosome 2. Genomics, 5(2), 291-297. https://doi.org/10.1016/0888-7543(89)90060-8