A glutaminase (Gls) gene maps to mouse chromosome 1, rat chromosome 9, and human chromosome 2

Beverly Mock, Christine Kozak, Michael F Seldin, Naomi Ruff, Lawrence D'Hoostelaere, Claude Szpirer, Hector Seuanez, Steve O'Brien, Carl Banner

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A rat cDNA clone encoding a portion of phosphate-activated glutaminase was used to identify DNA restriction fragment length polymorphisms (RFLPs) in sets of somatic cell hybrids and between wild-derived and inbred strains of mice. Segregation of rat and mouse chromosomes among somatic cell hybrids indicated assignment to rat chromosome 9 and mouse chromosome 1. Analysis of chromosome 1 alleles for several genes in an interspecific cross between Mus spretus and C3H HeJ- gld gld mice indicates that glutaminase can be positioned within 5.5 ± 2.0 cM proximal to Ctla-4. Similarly, human-hamster somatic cell hybrids were examined for RFLPs, and four human EcoRI restriction fragments were found to hybridize with the rat glutaminase probe. Two of these restriction fragments cosegregated and mapped to human chromosome 2 in a region that is syntenic with mouse chromosome 1 and rat chromosome 9.

Original languageEnglish (US)
Pages (from-to)291-297
Number of pages7
JournalGenomics
Volume5
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

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Glutaminase
Chromosomes, Human, Pair 9
Chromosomes, Human, Pair 2
Chromosomes, Human, Pair 1
Human Chromosomes
Hybrid Cells
Genes
Restriction Fragment Length Polymorphisms
Inbred Strains Mice
Cricetinae
Complementary DNA
Clone Cells
Chromosomes
Alleles
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

A glutaminase (Gls) gene maps to mouse chromosome 1, rat chromosome 9, and human chromosome 2. / Mock, Beverly; Kozak, Christine; Seldin, Michael F; Ruff, Naomi; D'Hoostelaere, Lawrence; Szpirer, Claude; Seuanez, Hector; O'Brien, Steve; Banner, Carl.

In: Genomics, Vol. 5, No. 2, 1989, p. 291-297.

Research output: Contribution to journalArticle

Mock, B, Kozak, C, Seldin, MF, Ruff, N, D'Hoostelaere, L, Szpirer, C, Seuanez, H, O'Brien, S & Banner, C 1989, 'A glutaminase (Gls) gene maps to mouse chromosome 1, rat chromosome 9, and human chromosome 2', Genomics, vol. 5, no. 2, pp. 291-297. https://doi.org/10.1016/0888-7543(89)90060-8
Mock, Beverly ; Kozak, Christine ; Seldin, Michael F ; Ruff, Naomi ; D'Hoostelaere, Lawrence ; Szpirer, Claude ; Seuanez, Hector ; O'Brien, Steve ; Banner, Carl. / A glutaminase (Gls) gene maps to mouse chromosome 1, rat chromosome 9, and human chromosome 2. In: Genomics. 1989 ; Vol. 5, No. 2. pp. 291-297.
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abstract = "A rat cDNA clone encoding a portion of phosphate-activated glutaminase was used to identify DNA restriction fragment length polymorphisms (RFLPs) in sets of somatic cell hybrids and between wild-derived and inbred strains of mice. Segregation of rat and mouse chromosomes among somatic cell hybrids indicated assignment to rat chromosome 9 and mouse chromosome 1. Analysis of chromosome 1 alleles for several genes in an interspecific cross between Mus spretus and C3H HeJ- gld gld mice indicates that glutaminase can be positioned within 5.5 ± 2.0 cM proximal to Ctla-4. Similarly, human-hamster somatic cell hybrids were examined for RFLPs, and four human EcoRI restriction fragments were found to hybridize with the rat glutaminase probe. Two of these restriction fragments cosegregated and mapped to human chromosome 2 in a region that is syntenic with mouse chromosome 1 and rat chromosome 9.",
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