A genetic map of the lettuce downy mildew pathogen, Bremia lactucae, constructed from molecular markers and avirulence genes

Delphine Sicard, Erik Legg, Sarah Brown, Nirmal K. Babu, Oswaldo Ochoa, Padma Sudarshana, Richard W Michelmore

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The genetic map of Bremia lactucae was expanded utilizing 97 F1 progeny derived from a cross between Finnish and Californian isolates (SF5 x C82P24). Genetic maps were constructed for each parent utilizing 7 avirulence genes, 83 RFLP markers, and 347 AFLP markers, and a consensus map was constructed from the complete data set. The framework map for SF5 contained 24 linkage groups distributed over 835cM; the map for C82P24 contained 21 linkage groups distributed over 606cM. The consensus map contained 12 linkage groups with markers from both parents and 24 parent-specific groups. Six avirulence genes mapped to different linkage groups; four were located at the ends of linkage groups. The closest linkages between molecular markers and avirulence genes were 3cM to Avr4 and 1cM to Avr7. Mating type seemed to be determined by a single locus, where the heterozygote determined the B2 type and the homozygous recessive genotype determined the B1 type.

Original languageEnglish (US)
Pages (from-to)16-30
Number of pages15
JournalFungal Genetics and Biology
Volume39
Issue number1
DOIs
StatePublished - Jun 2003

Fingerprint

Lettuce
Genes
Heterozygote
Restriction Fragment Length Polymorphisms
Genotype

Keywords

  • AFLP
  • Avirulence gene
  • Bremia lactucae
  • Downy mildew
  • Linkage map
  • Mating type
  • Oomycete
  • Plant pathogen
  • RFLP

ASJC Scopus subject areas

  • Genetics
  • Microbiology

Cite this

A genetic map of the lettuce downy mildew pathogen, Bremia lactucae, constructed from molecular markers and avirulence genes. / Sicard, Delphine; Legg, Erik; Brown, Sarah; Babu, Nirmal K.; Ochoa, Oswaldo; Sudarshana, Padma; Michelmore, Richard W.

In: Fungal Genetics and Biology, Vol. 39, No. 1, 06.2003, p. 16-30.

Research output: Contribution to journalArticle

Sicard, Delphine ; Legg, Erik ; Brown, Sarah ; Babu, Nirmal K. ; Ochoa, Oswaldo ; Sudarshana, Padma ; Michelmore, Richard W. / A genetic map of the lettuce downy mildew pathogen, Bremia lactucae, constructed from molecular markers and avirulence genes. In: Fungal Genetics and Biology. 2003 ; Vol. 39, No. 1. pp. 16-30.
@article{9ba5aaad915649889bb3d1ff74d49076,
title = "A genetic map of the lettuce downy mildew pathogen, Bremia lactucae, constructed from molecular markers and avirulence genes",
abstract = "The genetic map of Bremia lactucae was expanded utilizing 97 F1 progeny derived from a cross between Finnish and Californian isolates (SF5 x C82P24). Genetic maps were constructed for each parent utilizing 7 avirulence genes, 83 RFLP markers, and 347 AFLP markers, and a consensus map was constructed from the complete data set. The framework map for SF5 contained 24 linkage groups distributed over 835cM; the map for C82P24 contained 21 linkage groups distributed over 606cM. The consensus map contained 12 linkage groups with markers from both parents and 24 parent-specific groups. Six avirulence genes mapped to different linkage groups; four were located at the ends of linkage groups. The closest linkages between molecular markers and avirulence genes were 3cM to Avr4 and 1cM to Avr7. Mating type seemed to be determined by a single locus, where the heterozygote determined the B2 type and the homozygous recessive genotype determined the B1 type.",
keywords = "AFLP, Avirulence gene, Bremia lactucae, Downy mildew, Linkage map, Mating type, Oomycete, Plant pathogen, RFLP",
author = "Delphine Sicard and Erik Legg and Sarah Brown and Babu, {Nirmal K.} and Oswaldo Ochoa and Padma Sudarshana and Michelmore, {Richard W}",
year = "2003",
month = "6",
doi = "10.1016/S1087-1845(03)00005-7",
language = "English (US)",
volume = "39",
pages = "16--30",
journal = "Fungal Genetics and Biology",
issn = "1087-1845",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - A genetic map of the lettuce downy mildew pathogen, Bremia lactucae, constructed from molecular markers and avirulence genes

AU - Sicard, Delphine

AU - Legg, Erik

AU - Brown, Sarah

AU - Babu, Nirmal K.

AU - Ochoa, Oswaldo

AU - Sudarshana, Padma

AU - Michelmore, Richard W

PY - 2003/6

Y1 - 2003/6

N2 - The genetic map of Bremia lactucae was expanded utilizing 97 F1 progeny derived from a cross between Finnish and Californian isolates (SF5 x C82P24). Genetic maps were constructed for each parent utilizing 7 avirulence genes, 83 RFLP markers, and 347 AFLP markers, and a consensus map was constructed from the complete data set. The framework map for SF5 contained 24 linkage groups distributed over 835cM; the map for C82P24 contained 21 linkage groups distributed over 606cM. The consensus map contained 12 linkage groups with markers from both parents and 24 parent-specific groups. Six avirulence genes mapped to different linkage groups; four were located at the ends of linkage groups. The closest linkages between molecular markers and avirulence genes were 3cM to Avr4 and 1cM to Avr7. Mating type seemed to be determined by a single locus, where the heterozygote determined the B2 type and the homozygous recessive genotype determined the B1 type.

AB - The genetic map of Bremia lactucae was expanded utilizing 97 F1 progeny derived from a cross between Finnish and Californian isolates (SF5 x C82P24). Genetic maps were constructed for each parent utilizing 7 avirulence genes, 83 RFLP markers, and 347 AFLP markers, and a consensus map was constructed from the complete data set. The framework map for SF5 contained 24 linkage groups distributed over 835cM; the map for C82P24 contained 21 linkage groups distributed over 606cM. The consensus map contained 12 linkage groups with markers from both parents and 24 parent-specific groups. Six avirulence genes mapped to different linkage groups; four were located at the ends of linkage groups. The closest linkages between molecular markers and avirulence genes were 3cM to Avr4 and 1cM to Avr7. Mating type seemed to be determined by a single locus, where the heterozygote determined the B2 type and the homozygous recessive genotype determined the B1 type.

KW - AFLP

KW - Avirulence gene

KW - Bremia lactucae

KW - Downy mildew

KW - Linkage map

KW - Mating type

KW - Oomycete

KW - Plant pathogen

KW - RFLP

UR - http://www.scopus.com/inward/record.url?scp=0038668936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038668936&partnerID=8YFLogxK

U2 - 10.1016/S1087-1845(03)00005-7

DO - 10.1016/S1087-1845(03)00005-7

M3 - Article

C2 - 12742060

AN - SCOPUS:0038668936

VL - 39

SP - 16

EP - 30

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

SN - 1087-1845

IS - 1

ER -