A genetic characterization of the nadC gene of Salmonella typhimurium

K. T. Hughes, J. R. Roth, B. M. Olivera

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The nadC gene of Salmonella encodes the pyridine biosynthetic enzyme PRPP-quinolinate phosphoribosyltransferase. Using a combination of genetic techniques, a deletion map for the Salmonella nadC gene has been generated which includes over 100 point mutants and 18 deletion intervals. The nadC alleles obtained by hydroxylamine mutagenesis include those suppressed by either amber, ochre, or UGA nonsense suppressors as well as alleles suppressed by the missense suppressor, sumA. Deletions were obtained by three separate protocols including spontaneous selection for loss of the nearby aroP gene, recombination between aroP::MudA and nadC::MudA insertion alleles, and selection for spontaneous loss of tetracycline resistance in a nearby guaC::Tn10dTc insertion mutant allele. The nadC mutants comprise one complementation group and the nadC+ allele is dominant to simple, nadC auxotrophic mutant alleles. Intragenic complementation of two nadC alleles, nadC493 and nadC494, mapping to deletion intervals 17 and 18, respectively, suggests that nadC encodes a multimeric enzyme. Both nadC and the nearby aroP locus are transcribed counterclockwise on the standard genetic map of Salmonella, in opposite orientation to the direction of chromosome replication.

Original languageEnglish (US)
Pages (from-to)657-670
Number of pages14
JournalGenetics
Volume127
Issue number4
StatePublished - 1991
Externally publishedYes

Fingerprint

Salmonella typhimurium
Alleles
Genes
Salmonella
Amber
Tetracycline Resistance
Genetic Techniques
Hydroxylamine
Enzymes
Mutagenesis
Genetic Recombination
Chromosomes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Hughes, K. T., Roth, J. R., & Olivera, B. M. (1991). A genetic characterization of the nadC gene of Salmonella typhimurium. Genetics, 127(4), 657-670.

A genetic characterization of the nadC gene of Salmonella typhimurium. / Hughes, K. T.; Roth, J. R.; Olivera, B. M.

In: Genetics, Vol. 127, No. 4, 1991, p. 657-670.

Research output: Contribution to journalArticle

Hughes, KT, Roth, JR & Olivera, BM 1991, 'A genetic characterization of the nadC gene of Salmonella typhimurium', Genetics, vol. 127, no. 4, pp. 657-670.
Hughes KT, Roth JR, Olivera BM. A genetic characterization of the nadC gene of Salmonella typhimurium. Genetics. 1991;127(4):657-670.
Hughes, K. T. ; Roth, J. R. ; Olivera, B. M. / A genetic characterization of the nadC gene of Salmonella typhimurium. In: Genetics. 1991 ; Vol. 127, No. 4. pp. 657-670.
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