A general method for the purification of restriction enzymes

Patricia J. Greene, Herbert L. Heyneker, Francisco Bolivar, Raymond L. Rodriguez, Mary C. Betlach, Alejandra A. Covarrubias, Keith Backman, David J. Russel, Robert Tait, Herbert W. Boyer

Research output: Contribution to journalArticle

221 Scopus citations

Abstract

An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.

Original languageEnglish (US)
Pages (from-to)2373-2380
Number of pages8
JournalNucleic Acids Research
Volume5
Issue number7
DOIs
StatePublished - Jul 1978

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

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  • Cite this

    Greene, P. J., Heyneker, H. L., Bolivar, F., Rodriguez, R. L., Betlach, M. C., Covarrubias, A. A., Backman, K., Russel, D. J., Tait, R., & Boyer, H. W. (1978). A general method for the purification of restriction enzymes. Nucleic Acids Research, 5(7), 2373-2380. https://doi.org/10.1093/nar/5.7.2373