A general method for cloning DNA fragments in multiple copies

William H. Taylor; Paul J Hagerman

doi:10.1016/0378-1119(87)90001-1

A general method for cloning DNA fragments in multiple copies

William H. Taylor, Paul J Hagerman

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.

Original language	English (US)
Pages (from-to)	139-144
Number of pages	6
Journal	Gene
Volume	53
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1119(87)90001-1
State	Published - 1987
Externally published	Yes

Keywords

direct repeats
directional adapter
head-to-tail ligation
inserts
oligodeoxynucleotides
plasmid vector
Recombinant DNA
tandem repeats

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(87)90001-1

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title = "A general method for cloning DNA fragments in multiple copies",

abstract = "A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.",

keywords = "direct repeats, directional adapter, head-to-tail ligation, inserts, oligodeoxynucleotides, plasmid vector, Recombinant DNA, tandem repeats",

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year = "1987",

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language = "English (US)",

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pages = "139--144",

journal = "Gene",

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T1 - A general method for cloning DNA fragments in multiple copies

AU - Taylor, William H.

AU - Hagerman, Paul J

PY - 1987

Y1 - 1987

N2 - A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.

AB - A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.

KW - direct repeats

KW - directional adapter

KW - head-to-tail ligation

KW - inserts

KW - oligodeoxynucleotides

KW - plasmid vector

KW - Recombinant DNA

KW - tandem repeats

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AN - SCOPUS:0023218158

VL - 53

SP - 139

EP - 144

JO - Gene

JF - Gene

SN - 0378-1119

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