A general method for cloning DNA fragments in multiple copies

William H. Taylor, Paul J Hagerman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.

Original languageEnglish (US)
Pages (from-to)139-144
Number of pages6
JournalGene
Volume53
Issue number2-3
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Ligation
Organism Cloning
Nucleic Acid Repetitive Sequences
DNA
Plasmids
Clone Cells

Keywords

  • direct repeats
  • directional adapter
  • head-to-tail ligation
  • inserts
  • oligodeoxynucleotides
  • plasmid vector
  • Recombinant DNA
  • tandem repeats

ASJC Scopus subject areas

  • Genetics

Cite this

A general method for cloning DNA fragments in multiple copies. / Taylor, William H.; Hagerman, Paul J.

In: Gene, Vol. 53, No. 2-3, 1987, p. 139-144.

Research output: Contribution to journalArticle

Taylor, William H. ; Hagerman, Paul J. / A general method for cloning DNA fragments in multiple copies. In: Gene. 1987 ; Vol. 53, No. 2-3. pp. 139-144.
@article{72260fe849e94d66ac8685b6e6bde074,
title = "A general method for cloning DNA fragments in multiple copies",
abstract = "A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.",
keywords = "direct repeats, directional adapter, head-to-tail ligation, inserts, oligodeoxynucleotides, plasmid vector, Recombinant DNA, tandem repeats",
author = "Taylor, {William H.} and Hagerman, {Paul J}",
year = "1987",
doi = "10.1016/0378-1119(87)90001-1",
language = "English (US)",
volume = "53",
pages = "139--144",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - A general method for cloning DNA fragments in multiple copies

AU - Taylor, William H.

AU - Hagerman, Paul J

PY - 1987

Y1 - 1987

N2 - A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.

AB - A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.

KW - direct repeats

KW - directional adapter

KW - head-to-tail ligation

KW - inserts

KW - oligodeoxynucleotides

KW - plasmid vector

KW - Recombinant DNA

KW - tandem repeats

UR - http://www.scopus.com/inward/record.url?scp=0023218158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023218158&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(87)90001-1

DO - 10.1016/0378-1119(87)90001-1

M3 - Article

C2 - 3609745

AN - SCOPUS:0023218158

VL - 53

SP - 139

EP - 144

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2-3

ER -