A general method for cloning DNA fragments in multiple copies

William H. Taylor, Paul J Hagerman

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.

Original languageEnglish (US)
Pages (from-to)139-144
Number of pages6
JournalGene
Volume53
Issue number2-3
DOIs
StatePublished - 1987
Externally publishedYes

Keywords

  • direct repeats
  • directional adapter
  • head-to-tail ligation
  • inserts
  • oligodeoxynucleotides
  • plasmid vector
  • Recombinant DNA
  • tandem repeats

ASJC Scopus subject areas

  • Genetics

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