Abstract
A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 139-144 |
| Number of pages | 6 |
| Journal | Gene |
| Volume | 53 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - 1987 |
| Externally published | Yes |
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Keywords
- direct repeats
- directional adapter
- head-to-tail ligation
- inserts
- oligodeoxynucleotides
- plasmid vector
- Recombinant DNA
- tandem repeats
ASJC Scopus subject areas
- Genetics
Cite this
A general method for cloning DNA fragments in multiple copies. / Taylor, William H.; Hagerman, Paul J.
In: Gene, Vol. 53, No. 2-3, 1987, p. 139-144.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A general method for cloning DNA fragments in multiple copies
AU - Taylor, William H.
AU - Hagerman, Paul J
PY - 1987
Y1 - 1987
N2 - A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.
AB - A general method for the cloning of DNA fragments in tandem arrays is presented. Synthetic directional adapters are attached to the fragment ends to establish complete control over fragment orientation during ligation. Use of these directional adapters thereby ensures the production of direct repeats, an arrangement essential for clone stability. The technique involves a protocol that is both less complex and less time-onsuming than previous methods. Using this technique, a 10-min ligation has yielded a plasmid containing 20 copies of a 151-bp fragment.
KW - direct repeats
KW - directional adapter
KW - head-to-tail ligation
KW - inserts
KW - oligodeoxynucleotides
KW - plasmid vector
KW - Recombinant DNA
KW - tandem repeats
UR - http://www.scopus.com/inward/record.url?scp=0023218158&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023218158&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(87)90001-1
DO - 10.1016/0378-1119(87)90001-1
M3 - Article
VL - 53
SP - 139
EP - 144
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2-3
ER -