A general chemical synthesis platform for crosslinking multivalent single chain variable fragments

Joan G. Schellinger, Avinash Kudupudi, Arutselvan Natarajan, Wenjun Du, Sally J. Denardo, Jacquelyn Gervay-Hague

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Multivalent single chain variable fragments (scFv) show increased affinity to tumor-associated antigens compared to monovalent scFv and intact monoclonal antibodies (mAb). Multivalent constructs can be derived from self-associating or covalent scFv with covalent constructs offering improved in vivo and in vitro stability. Covalent attachment of scFv can be achieved using genetically engineered expression vectors that afford scFv with site specific cysteine functionality. Expression vectors for di-scFv-C wherein the cysteine is located in the center of two scFv have also been developed for attaching chemically reactive linkers. In the example illustrated here, the di-scFv-C is derived from a mAb directed against the MUC1 epitope, which is presented on cancer cells. To achieve multivalency, a chemical crosslinking strategy utilizing various azide and multi-alkyne functionalized polyethylene glycol (PEG) linkers was implemented. Conjugation was achieved by attachment of these linkers to the scFv thiol functionality. Chemoselective ligation was employed to covalently link different protein conjugates via copper(i) catalyzed azide alkyne 1,3-dipolar cycloaddition reaction (CuAAC) chemistry. Ligations were achieved in >70% yield using a specific set of linkers as determined by SDS-PAGE and densitometry. ELISA showed increased tumor binding of a tetravalent scFv providing a versatile chemical crosslinking strategy for construction of multivalent and bi-specific immunoconjugates that retain biological activity and have potential application in pre-targeted radioimmunotherapy and imaging.

Original languageEnglish (US)
Pages (from-to)1521-1526
Number of pages6
JournalOrganic and Biomolecular Chemistry
Volume10
Issue number8
DOIs
StatePublished - Feb 28 2012

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Single-Chain Antibodies
Alkynes
Azides
cysteine
alkynes
crosslinking
antibodies
Crosslinking
attachment
Cysteine
Ligation
Tumors
tumors
platforms
Monoclonal Antibodies
fragments
Immunoconjugates
Radioimmunotherapy
Cycloaddition
Densitometry

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Biochemistry

Cite this

A general chemical synthesis platform for crosslinking multivalent single chain variable fragments. / Schellinger, Joan G.; Kudupudi, Avinash; Natarajan, Arutselvan; Du, Wenjun; Denardo, Sally J.; Gervay-Hague, Jacquelyn.

In: Organic and Biomolecular Chemistry, Vol. 10, No. 8, 28.02.2012, p. 1521-1526.

Research output: Contribution to journalArticle

Schellinger, Joan G. ; Kudupudi, Avinash ; Natarajan, Arutselvan ; Du, Wenjun ; Denardo, Sally J. ; Gervay-Hague, Jacquelyn. / A general chemical synthesis platform for crosslinking multivalent single chain variable fragments. In: Organic and Biomolecular Chemistry. 2012 ; Vol. 10, No. 8. pp. 1521-1526.
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