A focused and efficient genetic screening strategy in the mouse

Identification of mutations that disrupt cortical development

Konstantinos Zarbalis, Scott R. May, Yiguo Shen, Marc Ekker, John L R Rubenstein, Andrew S. Peterson

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Although the mechanisms that regulate development of the cerebral cortex have begun to emerge, in large part through the analysis of mutant mice (Boncinelli et al. 2000; Molnar and Hannan 2000; Walsh and Goffinet 2000), many questions remain unanswered. To provide resources for further dissecting cortical development, we have carried out a focused screen for recessive mutations that disrupt cortical development. One aim of the screen was to identify mutants that disrupt the tangential migration of interneurons into the cortex. At the same time, we also screened for mutations that altered the growth or morphology of the cerebral cortex. We report here the identification of thirteen mutants with defects in aspects of cortical development ranging from the establishment of epithelial polarity to the invasion of thalamocortical axons. Among the collection are three novel alleles of genes for which mutant alleles had already been used to explore forebrain development, and four mutants with defects in interneuron migration. The mutants that we describe here will aid in deciphering the molecules and mechanisms that regulate cortical development. Our results also highlight the utility of focused screens in the mouse, in addition to the large-scale and broadly targeted screens that are being carried out at mutagenesis centers.

Original languageEnglish (US)
JournalPLoS Biology
Volume2
Issue number8
DOIs
StatePublished - Aug 2004
Externally publishedYes

Fingerprint

Genetic Testing
Interneurons
Cerebral Cortex
Screening
Alleles
screening
mutation
mutants
Mutagenesis
Defects
Mutation
mice
Prosencephalon
Axons
Genes
cerebral cortex
interneurons
Molecules
Growth
alleles

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

A focused and efficient genetic screening strategy in the mouse : Identification of mutations that disrupt cortical development. / Zarbalis, Konstantinos; May, Scott R.; Shen, Yiguo; Ekker, Marc; Rubenstein, John L R; Peterson, Andrew S.

In: PLoS Biology, Vol. 2, No. 8, 08.2004.

Research output: Contribution to journalArticle

Zarbalis, Konstantinos ; May, Scott R. ; Shen, Yiguo ; Ekker, Marc ; Rubenstein, John L R ; Peterson, Andrew S. / A focused and efficient genetic screening strategy in the mouse : Identification of mutations that disrupt cortical development. In: PLoS Biology. 2004 ; Vol. 2, No. 8.
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