A fluorescence lifetime spectroscopy study of matrix metalloproteinases -2 and -9 in human atherosclerotic plaque

Jennifer E. Phipps, Nisa Hatami, Zorina S. Galis, J. Dennis Baker, Michael C. Fishbein, Laura Marcu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Matrix metalloproteinase (MMP)-2 and -9 play important roles in the progression of atherosclerosis. This study aims to determine whether MMP-2 and -9 content in the fibrotic caps of atherosclerotic plaque is correlated with plaque autofluorescence. A time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) system was used to measure the autofluorescence and assess the biochemical composition of human plaques obtained from carotid endarterectomy. Results presented here demonstrate for the first time the ability to characterize the biochemical composition as it relates to MMP-2 and -9 content in the atherosclerotic plaque cap using a label-free imaging technique implemented with a fiberoptic TR-LIFS system.

Original languageEnglish (US)
Pages (from-to)650-658
Number of pages9
JournalJournal of Biophotonics
Volume4
Issue number9
DOIs
StatePublished - Sep 2011

Fingerprint

Matrix Metalloproteinase 2
Fluorescence Spectrometry
Matrix Metalloproteinase 9
Atherosclerotic Plaques
Fluorescence
Fluorescence spectroscopy
Spectroscopy
caps
life (durability)
fluorescence
laser induced fluorescence
Lasers
matrices
spectroscopy
arteriosclerosis
Carotid Endarterectomy
Chemical analysis
progressions
imaging techniques
Labels

Keywords

  • Atherosclerosis
  • Carotid plaque
  • Matrix metalloproteinases
  • Time-resolved fluorescence spectroscopy

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A fluorescence lifetime spectroscopy study of matrix metalloproteinases -2 and -9 in human atherosclerotic plaque. / Phipps, Jennifer E.; Hatami, Nisa; Galis, Zorina S.; Baker, J. Dennis; Fishbein, Michael C.; Marcu, Laura.

In: Journal of Biophotonics, Vol. 4, No. 9, 09.2011, p. 650-658.

Research output: Contribution to journalArticle

Phipps, Jennifer E. ; Hatami, Nisa ; Galis, Zorina S. ; Baker, J. Dennis ; Fishbein, Michael C. ; Marcu, Laura. / A fluorescence lifetime spectroscopy study of matrix metalloproteinases -2 and -9 in human atherosclerotic plaque. In: Journal of Biophotonics. 2011 ; Vol. 4, No. 9. pp. 650-658.
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