Abstract
Matrix metalloproteinase (MMP)-2 and -9 play important roles in the progression of atherosclerosis. This study aims to determine whether MMP-2 and -9 content in the fibrotic caps of atherosclerotic plaque is correlated with plaque autofluorescence. A time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) system was used to measure the autofluorescence and assess the biochemical composition of human plaques obtained from carotid endarterectomy. Results presented here demonstrate for the first time the ability to characterize the biochemical composition as it relates to MMP-2 and -9 content in the atherosclerotic plaque cap using a label-free imaging technique implemented with a fiberoptic TR-LIFS system.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 650-658 |
| Number of pages | 9 |
| Journal | Journal of Biophotonics |
| Volume | 4 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2011 |
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Keywords
- Atherosclerosis
- Carotid plaque
- Matrix metalloproteinases
- Time-resolved fluorescence spectroscopy
ASJC Scopus subject areas
- Materials Science(all)
- Engineering(all)
- Physics and Astronomy(all)
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
A fluorescence lifetime spectroscopy study of matrix metalloproteinases -2 and -9 in human atherosclerotic plaque. / Phipps, Jennifer E.; Hatami, Nisa; Galis, Zorina S.; Baker, J. Dennis; Fishbein, Michael C.; Marcu, Laura.
In: Journal of Biophotonics, Vol. 4, No. 9, 09.2011, p. 650-658.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A fluorescence lifetime spectroscopy study of matrix metalloproteinases -2 and -9 in human atherosclerotic plaque
AU - Phipps, Jennifer E.
AU - Hatami, Nisa
AU - Galis, Zorina S.
AU - Baker, J. Dennis
AU - Fishbein, Michael C.
AU - Marcu, Laura
PY - 2011/9
Y1 - 2011/9
N2 - Matrix metalloproteinase (MMP)-2 and -9 play important roles in the progression of atherosclerosis. This study aims to determine whether MMP-2 and -9 content in the fibrotic caps of atherosclerotic plaque is correlated with plaque autofluorescence. A time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) system was used to measure the autofluorescence and assess the biochemical composition of human plaques obtained from carotid endarterectomy. Results presented here demonstrate for the first time the ability to characterize the biochemical composition as it relates to MMP-2 and -9 content in the atherosclerotic plaque cap using a label-free imaging technique implemented with a fiberoptic TR-LIFS system.
AB - Matrix metalloproteinase (MMP)-2 and -9 play important roles in the progression of atherosclerosis. This study aims to determine whether MMP-2 and -9 content in the fibrotic caps of atherosclerotic plaque is correlated with plaque autofluorescence. A time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) system was used to measure the autofluorescence and assess the biochemical composition of human plaques obtained from carotid endarterectomy. Results presented here demonstrate for the first time the ability to characterize the biochemical composition as it relates to MMP-2 and -9 content in the atherosclerotic plaque cap using a label-free imaging technique implemented with a fiberoptic TR-LIFS system.
KW - Atherosclerosis
KW - Carotid plaque
KW - Matrix metalloproteinases
KW - Time-resolved fluorescence spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=80051950946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051950946&partnerID=8YFLogxK
U2 - 10.1002/jbio.201100042
DO - 10.1002/jbio.201100042
M3 - Article
C2 - 21770037
AN - SCOPUS:80051950946
VL - 4
SP - 650
EP - 658
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 9
ER -