A fluorescence lifetime spectroscopy study of matrix metalloproteinases -2 and -9 in human atherosclerotic plaque

Jennifer E. Phipps; Nisa Hatami; Zorina S. Galis; J. Dennis Baker; Michael C. Fishbein; Laura Marcu

doi:10.1002/jbio.201100042

A fluorescence lifetime spectroscopy study of matrix metalloproteinases -2 and -9 in human atherosclerotic plaque

Jennifer E. Phipps, Nisa Hatami, Zorina S. Galis, J. Dennis Baker, Michael C. Fishbein, Laura Marcu

Neurological Surgery

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Matrix metalloproteinase (MMP)-2 and -9 play important roles in the progression of atherosclerosis. This study aims to determine whether MMP-2 and -9 content in the fibrotic caps of atherosclerotic plaque is correlated with plaque autofluorescence. A time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) system was used to measure the autofluorescence and assess the biochemical composition of human plaques obtained from carotid endarterectomy. Results presented here demonstrate for the first time the ability to characterize the biochemical composition as it relates to MMP-2 and -9 content in the atherosclerotic plaque cap using a label-free imaging technique implemented with a fiberoptic TR-LIFS system.

Original language	English (US)
Pages (from-to)	650-658
Number of pages	9
Journal	Journal of Biophotonics
Volume	4
Issue number	9
DOIs	https://doi.org/10.1002/jbio.201100042
State	Published - Sep 2011

Keywords

Atherosclerosis
Carotid plaque
Matrix metalloproteinases
Time-resolved fluorescence spectroscopy

ASJC Scopus subject areas

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)

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10.1002/jbio.201100042

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title = "A fluorescence lifetime spectroscopy study of matrix metalloproteinases -2 and -9 in human atherosclerotic plaque",

abstract = "Matrix metalloproteinase (MMP)-2 and -9 play important roles in the progression of atherosclerosis. This study aims to determine whether MMP-2 and -9 content in the fibrotic caps of atherosclerotic plaque is correlated with plaque autofluorescence. A time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) system was used to measure the autofluorescence and assess the biochemical composition of human plaques obtained from carotid endarterectomy. Results presented here demonstrate for the first time the ability to characterize the biochemical composition as it relates to MMP-2 and -9 content in the atherosclerotic plaque cap using a label-free imaging technique implemented with a fiberoptic TR-LIFS system.",

keywords = "Atherosclerosis, Carotid plaque, Matrix metalloproteinases, Time-resolved fluorescence spectroscopy",

author = "Phipps, {Jennifer E.} and Nisa Hatami and Galis, {Zorina S.} and Baker, {J. Dennis} and Fishbein, {Michael C.} and Laura Marcu",

year = "2011",

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AU - Phipps, Jennifer E.

AU - Hatami, Nisa

AU - Galis, Zorina S.

AU - Baker, J. Dennis

AU - Fishbein, Michael C.

AU - Marcu, Laura

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AB - Matrix metalloproteinase (MMP)-2 and -9 play important roles in the progression of atherosclerosis. This study aims to determine whether MMP-2 and -9 content in the fibrotic caps of atherosclerotic plaque is correlated with plaque autofluorescence. A time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) system was used to measure the autofluorescence and assess the biochemical composition of human plaques obtained from carotid endarterectomy. Results presented here demonstrate for the first time the ability to characterize the biochemical composition as it relates to MMP-2 and -9 content in the atherosclerotic plaque cap using a label-free imaging technique implemented with a fiberoptic TR-LIFS system.

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