@inbook{d452bcc0d7c3425295e9a386e2a32f5b,
title = "A FLIPR assay for evaluating agonists and antagonists of GPCR heterodimers",
abstract = "Calcium signaling plays a major role in the function of cells. Measurement of intracellular calcium mobilization is a robust assay that can be performed in a high-throughput manner to study the effect of compounds on potential drug targets. Pharmaceutical companies frequently use calcium signaling assays to screen compound libraries on G-protein-coupled receptors (GPCRs). In this chapter we describe the application of FLIPR technology to the evaluation of GPCR-induced calcium mobilization. We also include the implications of GPCR hetero-oligomerization and the identification of heteromeric receptors as novel drug targets on high-throughput calcium screening.",
keywords = "Bivalent ligands, Calcium mobilization, G-protein-coupled receptor, GPCR, Heteromer, High-throughput screening, HTS, Oligomerization",
author = "Harvey, {Jessica H.} and {Van Rijn}, {Richard M.} and Jennifer Whistler",
year = "2013",
month = may,
day = "8",
doi = "10.1007/978-1-62703-345-9_4",
language = "English (US)",
isbn = "9781627033442",
series = "Methods in Molecular Biology",
pages = "43--54",
booktitle = "Chemical Neurobiology",
}