A filter paper sandwich method using small volumes of reagents for the detection of antigens electrophoretically transferred onto nitrocellulose

Gordon C Douglas, Barry F. King

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A method is described which allows antigens electrophoretically 'blotted' onto nitrocellulose to be probed using small volumes (<0.3 ml) of antibody solutions. Reagent volumes required for this method are more than 10-fold lower than the minimum volumes attainable using either custom made or commercial incubation trays. The procedure is simple and economical and should be applicable to any situation where it is desirable to either conserve or reduce the amount of screening reagent used.

Original languageEnglish (US)
Pages (from-to)333-338
Number of pages6
JournalJournal of Immunological Methods
Volume75
Issue number2
DOIs
StatePublished - Dec 31 1984

Keywords

  • antigenic analysis
  • immunoblotting
  • transferrin receptor

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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