A feline immunodeficiency virus vif-deletion mutant remains attenuated upon infection of newborn kittens

Xiaoying Shen, Christian M. Leutenegger, Kelly Stefano Cole, Niels C Pedersen, Ellen E. Sparger

Research output: Contribution to journalArticle

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Abstract

This report characterizes lentivirus attenuation associated with a vif mutation by inoculation of newborn kittens with a vif-deleted feline immunodeficiency virus provirus plasmid (FIV-pPPRΔvif). Virus in peripheral blood, antiviral antibody or CD4 T-cell count alterations were not detected in kittens inoculated with FIV-pPPRΔvif plasmid, with the exception of one kitten that demonstrated FIV Gag antibody production at 42 weeks after inoculation. In contrast, wild-type FIV-pPPR-infected kittens were viraemic, seropositive and exhibited a decrease in the CD4 T-cell subset in peripheral blood. Interestingly, FIV-specific T-cell proliferative responses detected at 32 and 36 weeks after infection were comparable for both FIV-pPPRΔvif- and wild-type FIV-pPPR-inoculated kittens and suggested the possibility of a discreet tissue reservoir supporting sustained FIV-pPPRΔvif expression or replication. Overall, these findings confirmed that the severe virus attenuation for both replication and pathogenicity exhibited by a vif-deleted FIV mutant is similar for both neonatal and adult hosts.

Original languageEnglish (US)
Pages (from-to)2793-2799
Number of pages7
JournalJournal of General Virology
Volume88
Issue number10
DOIs
StatePublished - Oct 2007

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Feline Immunodeficiency Virus
Infection
Plasmids
Viruses
T-Lymphocytes
Proviruses
Lentivirus
T-Lymphocyte Subsets
CD4 Lymphocyte Count
Antibody Formation
Antiviral Agents
Virulence

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

A feline immunodeficiency virus vif-deletion mutant remains attenuated upon infection of newborn kittens. / Shen, Xiaoying; Leutenegger, Christian M.; Cole, Kelly Stefano; Pedersen, Niels C; Sparger, Ellen E.

In: Journal of General Virology, Vol. 88, No. 10, 10.2007, p. 2793-2799.

Research output: Contribution to journalArticle

Shen, Xiaoying ; Leutenegger, Christian M. ; Cole, Kelly Stefano ; Pedersen, Niels C ; Sparger, Ellen E. / A feline immunodeficiency virus vif-deletion mutant remains attenuated upon infection of newborn kittens. In: Journal of General Virology. 2007 ; Vol. 88, No. 10. pp. 2793-2799.
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