A dual vacuum chamber fourier transform mass spectrometer with rapidly interchangeable LSIMS, MALDI, and ESI sources: Initial results with LSIMS and MALDI

James A. Carroll, Sharron G. Penn, Steven T. Fannin, Jiangyue Wu, Mark T. Cancilla, M. Kirk Green, Carlito B Lebrilla

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A design is presented involving two separate vacuum chambers to provide nearly simultaneous capabilities of liquid secondary ion mass spectrometry (LSIMS), matrix-assisted laser desorption/ionization (MALDI), and electrospray ionization (ESI) in an external source Fourier transform mass spectrometer. The instrument consists of two vacuum chambers, one with five stages of differential pumping for ESI and another with two stages of differential pumping for a combined LSIMS/MALDI source. The chamber dedicated to ESI was formerly a three-stage chamber with LSIMS and electron ionization. Two additional stages were added with the ESI source. LSIMS and MALDI have similar vacuum requirements and were moved to a newly built chamber with two stages of pumping. We present our first results obtained on the new vacuum chamber. Data presented for the MALDI source show that, with only two stages of pumping, and with shorter radio frequency-only quadrupole rods for ion injection, spectra comparable to those obtained on the formerly three-stage instrument can be obtained. Characterization of the MALDI source and data on linear, cyclic, and branched oligosaccharides are given. Finally, the design of the second chamber is proposed as a low-cost prototype for an external source FTMS instrument.

Original languageEnglish (US)
Pages (from-to)1798-1804
Number of pages7
JournalAnalytical Chemistry
Volume68
Issue number10
StatePublished - 1996

ASJC Scopus subject areas

  • Analytical Chemistry

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