Abstract
A versatile, inexpensive electrospray interface has been constructed and installed on an external source Fourier transform mass spectrometer with a quadrupole ion guide. This interface is readily interchangeable with FAB and MALDI sources, with the main time requirement for changing being the pump-down time. A heated capillary, two skimmers and a set of Einzel lenses are used to deliver ions to the quadrupole ion guide. SIMION (Idaho National Engineering Laboratory, Idaho Falls, Idaho) was used to model ion trajectories and determine optimal dimensions. Five stages of pumping are employed, using mechanical, turbomolecular and cryopumps. A solenoid-driven shutter on the final lens blocks neutrals after injection of the ion packet. Kinetic energy distributions of both small and large biomolecular ions were investigated. For large (104-105 Da), highly charged species, the kinetic energy distributions were quite favorable to trapping, with sharp peaks at less than 10 eV. Thus a 50 ms ion accumulation time is sufficient to obtain spectra of lysozyme with high S/N ratio. Distributions for smaller ions tended to be somewhat wider and peaked at higher energies. The spectra obtained to date illustrate the ability of the instrument to handle biomolecules ranging from bradykinin to bovine serum albumen.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 115-127 |
| Number of pages | 13 |
| Journal | International Journal of Mass Spectrometry and Ion Processes |
| Volume | 157-158 |
| State | Published - 1996 |
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Keywords
- Collision induced dissociation
- Electrospray
- Fourier transform mass spectrometry
- Instrumentation
- Kinetic energy
ASJC Scopus subject areas
- Spectroscopy
Cite this
A dual vacuum chamber Fourier transform mass spectrometer with rapidly interchangeable FAB, MALDI and ESI sources : Electrospray results. / Gard, Eric E.; Green, M. Kirk; Warren, Harris; Camara, Elvira J O; He, Fei; Penn, Sharron G.; Lebrilla, Carlito B.
In: International Journal of Mass Spectrometry and Ion Processes, Vol. 157-158, 1996, p. 115-127.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A dual vacuum chamber Fourier transform mass spectrometer with rapidly interchangeable FAB, MALDI and ESI sources
T2 - Electrospray results
AU - Gard, Eric E.
AU - Green, M. Kirk
AU - Warren, Harris
AU - Camara, Elvira J O
AU - He, Fei
AU - Penn, Sharron G.
AU - Lebrilla, Carlito B
PY - 1996
Y1 - 1996
N2 - A versatile, inexpensive electrospray interface has been constructed and installed on an external source Fourier transform mass spectrometer with a quadrupole ion guide. This interface is readily interchangeable with FAB and MALDI sources, with the main time requirement for changing being the pump-down time. A heated capillary, two skimmers and a set of Einzel lenses are used to deliver ions to the quadrupole ion guide. SIMION (Idaho National Engineering Laboratory, Idaho Falls, Idaho) was used to model ion trajectories and determine optimal dimensions. Five stages of pumping are employed, using mechanical, turbomolecular and cryopumps. A solenoid-driven shutter on the final lens blocks neutrals after injection of the ion packet. Kinetic energy distributions of both small and large biomolecular ions were investigated. For large (104-105 Da), highly charged species, the kinetic energy distributions were quite favorable to trapping, with sharp peaks at less than 10 eV. Thus a 50 ms ion accumulation time is sufficient to obtain spectra of lysozyme with high S/N ratio. Distributions for smaller ions tended to be somewhat wider and peaked at higher energies. The spectra obtained to date illustrate the ability of the instrument to handle biomolecules ranging from bradykinin to bovine serum albumen.
AB - A versatile, inexpensive electrospray interface has been constructed and installed on an external source Fourier transform mass spectrometer with a quadrupole ion guide. This interface is readily interchangeable with FAB and MALDI sources, with the main time requirement for changing being the pump-down time. A heated capillary, two skimmers and a set of Einzel lenses are used to deliver ions to the quadrupole ion guide. SIMION (Idaho National Engineering Laboratory, Idaho Falls, Idaho) was used to model ion trajectories and determine optimal dimensions. Five stages of pumping are employed, using mechanical, turbomolecular and cryopumps. A solenoid-driven shutter on the final lens blocks neutrals after injection of the ion packet. Kinetic energy distributions of both small and large biomolecular ions were investigated. For large (104-105 Da), highly charged species, the kinetic energy distributions were quite favorable to trapping, with sharp peaks at less than 10 eV. Thus a 50 ms ion accumulation time is sufficient to obtain spectra of lysozyme with high S/N ratio. Distributions for smaller ions tended to be somewhat wider and peaked at higher energies. The spectra obtained to date illustrate the ability of the instrument to handle biomolecules ranging from bradykinin to bovine serum albumen.
KW - Collision induced dissociation
KW - Electrospray
KW - Fourier transform mass spectrometry
KW - Instrumentation
KW - Kinetic energy
UR - http://www.scopus.com/inward/record.url?scp=0000091225&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0000091225&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0000091225
VL - 157-158
SP - 115
EP - 127
JO - International Journal of Mass Spectrometry
JF - International Journal of Mass Spectrometry
SN - 1387-3806
ER -