A dual selection module for directed evolution of genetic circuits

Yohei Yokobayashi, Frances H. Arnold

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We have developed a genetic selection module which enables us to select for positive and negative expression of a gene by controlling the expression of two antibiotic resistance genes. This module can be used to tune the performance of genetic circuits that yield simple ON/OFF gene expression outputs depending on the environment (input). We have demonstrated the utility of the module by selecting a genetic inverter from a 200 fold excess of nonfunctional inverters in two rounds.

Original languageEnglish (US)
Pages (from-to)245-254
Number of pages10
JournalNatural Computing
Volume4
Issue number3
DOIs
StatePublished - Sep 2005

Keywords

  • Cellular computation
  • Directed evolution
  • Genetic circuits
  • Selection
  • Synthetic biology
  • Synthetic gene circuits

ASJC Scopus subject areas

  • Computer Science(all)

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