A dog model to study ovary, ovarian ligament and visceral pain

Pedro Boscan, Eric Monnet, Khursheed Mama, David C. Twedt, Jonathan Congdon, Jens C. Eickhoff, Eugene Steffey

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Objective A dog model was developed to study visceral pain by stimulating the ovarian ligament. Study design Prospective experimental trial. Animals Twelve 1-year old female hound dogs weighing 25.7wplusmn;3.6kg. Methods Dogs were anesthetized with sevoflurane. The right ovary was accessed via laparoscopy. A suture was placed around the ovarian ligament and exteriorized through the abdominal wall for stimulation. The noxious stimulus consisted of pulling the ovary and ovarian ligament with a force transducer. The response to noxious stimulation was determined using the anesthetic minimum alveolar concentration requirement (MAC) for sevoflurane. The ovarian MAC was compared to the standardized somatic noxious stimulation tail clamp MAC. The results are depicted as mean±SD and corrected to sea-level. Results The stimulus-response curve during ovarian stimulation in three dogs was hyperbolic and best represented by a three-parameter logistic growth curve model. The curve plateaued at 7.12±4.19N. From the stimulus-response curve, we chose 6.61N to test the consistency and repeatability of the model in nine dogs. The ovarian stimulation MAC for sevoflurane in these dogs was 2.16±0.46%. The ovarian stimulation confidence interval and limits are comparable to the results from tail stimulation MAC. The tail stimulation MACs before and after laparoscopy surgery were not different (1.86± 0.28% and 1.77±0.38% respectively; p>0.05) but lower when compared to the ovarian MAC (p<0.01). The dogs recovered from anesthesia without complications. Conclusions and clinical relevance The ovarian stimulation model is an adequate and repeatable means of producing visceral stimulation to determine MAC. The model may provide a humane mechanism to study the effectiveness of analgesics for acute ovarian pain.

Original languageEnglish (US)
Pages (from-to)260-266
Number of pages7
JournalVeterinary Anaesthesia and Analgesia
Volume38
Issue number3
DOIs
StatePublished - Jan 1 2011

Fingerprint

Visceral Pain
ligaments
Ligaments
pain
Ovary
Dogs
Ovulation Induction
dogs
Tail
laparoscopy
tail
Laparoscopy
hounds
transducers (equipment)
Acute Pain
Abdominal Wall
analgesics
sutures
Transducers
Oceans and Seas

Keywords

  • Ovary
  • Reproductive tract
  • Spay
  • Visceral pain

ASJC Scopus subject areas

  • veterinary(all)

Cite this

A dog model to study ovary, ovarian ligament and visceral pain. / Boscan, Pedro; Monnet, Eric; Mama, Khursheed; Twedt, David C.; Congdon, Jonathan; Eickhoff, Jens C.; Steffey, Eugene.

In: Veterinary Anaesthesia and Analgesia, Vol. 38, No. 3, 01.01.2011, p. 260-266.

Research output: Contribution to journalArticle

Boscan, P, Monnet, E, Mama, K, Twedt, DC, Congdon, J, Eickhoff, JC & Steffey, E 2011, 'A dog model to study ovary, ovarian ligament and visceral pain', Veterinary Anaesthesia and Analgesia, vol. 38, no. 3, pp. 260-266. https://doi.org/10.1111/j.1467-2995.2011.00611.x
Boscan P, Monnet E, Mama K, Twedt DC, Congdon J, Eickhoff JC et al. A dog model to study ovary, ovarian ligament and visceral pain. Veterinary Anaesthesia and Analgesia. 2011 Jan 1;38(3):260-266. https://doi.org/10.1111/j.1467-2995.2011.00611.x
Boscan, Pedro ; Monnet, Eric ; Mama, Khursheed ; Twedt, David C. ; Congdon, Jonathan ; Eickhoff, Jens C. ; Steffey, Eugene. / A dog model to study ovary, ovarian ligament and visceral pain. In: Veterinary Anaesthesia and Analgesia. 2011 ; Vol. 38, No. 3. pp. 260-266.
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abstract = "Objective A dog model was developed to study visceral pain by stimulating the ovarian ligament. Study design Prospective experimental trial. Animals Twelve 1-year old female hound dogs weighing 25.7wplusmn;3.6kg. Methods Dogs were anesthetized with sevoflurane. The right ovary was accessed via laparoscopy. A suture was placed around the ovarian ligament and exteriorized through the abdominal wall for stimulation. The noxious stimulus consisted of pulling the ovary and ovarian ligament with a force transducer. The response to noxious stimulation was determined using the anesthetic minimum alveolar concentration requirement (MAC) for sevoflurane. The ovarian MAC was compared to the standardized somatic noxious stimulation tail clamp MAC. The results are depicted as mean±SD and corrected to sea-level. Results The stimulus-response curve during ovarian stimulation in three dogs was hyperbolic and best represented by a three-parameter logistic growth curve model. The curve plateaued at 7.12±4.19N. From the stimulus-response curve, we chose 6.61N to test the consistency and repeatability of the model in nine dogs. The ovarian stimulation MAC for sevoflurane in these dogs was 2.16±0.46{\%}. The ovarian stimulation confidence interval and limits are comparable to the results from tail stimulation MAC. The tail stimulation MACs before and after laparoscopy surgery were not different (1.86± 0.28{\%} and 1.77±0.38{\%} respectively; p>0.05) but lower when compared to the ovarian MAC (p<0.01). The dogs recovered from anesthesia without complications. Conclusions and clinical relevance The ovarian stimulation model is an adequate and repeatable means of producing visceral stimulation to determine MAC. The model may provide a humane mechanism to study the effectiveness of analgesics for acute ovarian pain.",
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