Abstract
The cytotoxicity of bifunctional alkylating agents is generally attributed to DNA damage, especially DNA-DNA crosslinking activity. It is unclear how crosslinks or other cellular damage result in cell death. Studies of drug effects at the level of expression of specific gene products may help elucidate the mechanism of cell killing. We examined proteins synthesized in L-phenylalanine mustard treated human lymphoma cells by [35S]methionine labeling and SDS-PAGE. Drug-treated cells showed decreased labeling of proteins in two molecular weight bands of 17 kDa (a doublet) and 12 kDa at 6, 18 and 24 hours after drug removal. One of the components of the 17 kDa doublet has been identified as calmodulin, a calcium binding protein essential to cell cycle progression and survival.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 717-721 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 176 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 30 1991 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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A DNA crosslinking drug alters synthesis of several low molecular weight proteins in human lymphoma cells. / Widstrom, Richard L.; Ducore, Jonathan M.
In: Biochemical and Biophysical Research Communications, Vol. 176, No. 2, 30.04.1991, p. 717-721.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A DNA crosslinking drug alters synthesis of several low molecular weight proteins in human lymphoma cells
AU - Widstrom, Richard L.
AU - Ducore, Jonathan M
PY - 1991/4/30
Y1 - 1991/4/30
N2 - The cytotoxicity of bifunctional alkylating agents is generally attributed to DNA damage, especially DNA-DNA crosslinking activity. It is unclear how crosslinks or other cellular damage result in cell death. Studies of drug effects at the level of expression of specific gene products may help elucidate the mechanism of cell killing. We examined proteins synthesized in L-phenylalanine mustard treated human lymphoma cells by [35S]methionine labeling and SDS-PAGE. Drug-treated cells showed decreased labeling of proteins in two molecular weight bands of 17 kDa (a doublet) and 12 kDa at 6, 18 and 24 hours after drug removal. One of the components of the 17 kDa doublet has been identified as calmodulin, a calcium binding protein essential to cell cycle progression and survival.
AB - The cytotoxicity of bifunctional alkylating agents is generally attributed to DNA damage, especially DNA-DNA crosslinking activity. It is unclear how crosslinks or other cellular damage result in cell death. Studies of drug effects at the level of expression of specific gene products may help elucidate the mechanism of cell killing. We examined proteins synthesized in L-phenylalanine mustard treated human lymphoma cells by [35S]methionine labeling and SDS-PAGE. Drug-treated cells showed decreased labeling of proteins in two molecular weight bands of 17 kDa (a doublet) and 12 kDa at 6, 18 and 24 hours after drug removal. One of the components of the 17 kDa doublet has been identified as calmodulin, a calcium binding protein essential to cell cycle progression and survival.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025844840&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(05)80243-8
DO - 10.1016/S0006-291X(05)80243-8
M3 - Article
C2 - 2025284
AN - SCOPUS:0025844840
VL - 176
SP - 717
EP - 721
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -