A distinct pathway of cell-mediated apoptosis initiated by Granulysin

A. A. Kaspar, S. Okada, J. Kumar, Francis R Poulain, K. A. Drouvalakis, A. Kelekar, D. A. Hanson, R. M. Kluck, Y. Hitoshi, D. E. Johnson, C. J. Froelich, C. B. Thompson, D. D. Newmeyer, A. Anel, C. Clayberger, A. M. Krensky

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Granulysin is an antimicrobial and tumoricidal molecule expressed in granules of CTL and NK cells. In this study, we show that granulysin damages cell membranes based upon negative charge, disrupts the transmembrane potential (Δψ) in mitochondria, and causes release of cytochrome c. Granulysin-induced apoptosis is blocked in cells overexpressing Bcl-2. Despite the release of cytochrome c, procaspase 9 is not processed. Nevertheless, activation of caspase 3 is observed in granulysin-treated cells, suggesting that granulysin activates a novel pathway of CTL- and NK cell-mediated death distinct from granzyme- and death receptor-induced apoptosis.

Original languageEnglish (US)
Pages (from-to)350-356
Number of pages7
JournalJournal of Immunology
Volume167
Issue number1
StatePublished - Jul 1 2001
Externally publishedYes

Fingerprint

Cytochromes c
Natural Killer Cells
Apoptosis
Granzymes
Death Domain Receptors
Caspase 9
Caspase 3
Membrane Potentials
Mitochondria
Cell Death
Cell Membrane

ASJC Scopus subject areas

  • Immunology

Cite this

Kaspar, A. A., Okada, S., Kumar, J., Poulain, F. R., Drouvalakis, K. A., Kelekar, A., ... Krensky, A. M. (2001). A distinct pathway of cell-mediated apoptosis initiated by Granulysin. Journal of Immunology, 167(1), 350-356.

A distinct pathway of cell-mediated apoptosis initiated by Granulysin. / Kaspar, A. A.; Okada, S.; Kumar, J.; Poulain, Francis R; Drouvalakis, K. A.; Kelekar, A.; Hanson, D. A.; Kluck, R. M.; Hitoshi, Y.; Johnson, D. E.; Froelich, C. J.; Thompson, C. B.; Newmeyer, D. D.; Anel, A.; Clayberger, C.; Krensky, A. M.

In: Journal of Immunology, Vol. 167, No. 1, 01.07.2001, p. 350-356.

Research output: Contribution to journalArticle

Kaspar, AA, Okada, S, Kumar, J, Poulain, FR, Drouvalakis, KA, Kelekar, A, Hanson, DA, Kluck, RM, Hitoshi, Y, Johnson, DE, Froelich, CJ, Thompson, CB, Newmeyer, DD, Anel, A, Clayberger, C & Krensky, AM 2001, 'A distinct pathway of cell-mediated apoptosis initiated by Granulysin', Journal of Immunology, vol. 167, no. 1, pp. 350-356.
Kaspar AA, Okada S, Kumar J, Poulain FR, Drouvalakis KA, Kelekar A et al. A distinct pathway of cell-mediated apoptosis initiated by Granulysin. Journal of Immunology. 2001 Jul 1;167(1):350-356.
Kaspar, A. A. ; Okada, S. ; Kumar, J. ; Poulain, Francis R ; Drouvalakis, K. A. ; Kelekar, A. ; Hanson, D. A. ; Kluck, R. M. ; Hitoshi, Y. ; Johnson, D. E. ; Froelich, C. J. ; Thompson, C. B. ; Newmeyer, D. D. ; Anel, A. ; Clayberger, C. ; Krensky, A. M. / A distinct pathway of cell-mediated apoptosis initiated by Granulysin. In: Journal of Immunology. 2001 ; Vol. 167, No. 1. pp. 350-356.
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