A decrease in intracellular zinc level precedes the detection of early indicators of apoptosis in HL-60 cells

J. Y. Duffy, C. M. Miller, G. L. Rutschilling, G. M. Ridder, M. S. Clegg, Carl L Keen, G. P. Daston

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Low extracellular zinc concentrations have been associated with the induction of apoptosis. To assess the relationship between intracellular zinc concentration and the rate of apoptosis, cells were grown in media containing 0.5, 25, or 50 μM zinc and analyzed by flow cytometry or fluorescence microscopy. Cells grown in 0.5 μM zinc medium over 48 h showed a successive decrease in intracellular zinc concentration measured by the zinc-specific fluorophore, zinquin. After 18 h in 0.5 μM zinc medium, rhodamine 123 retention decreased. However, the addition of 10 μM zinc to the 0.5 μM medium before 16 h in culture restored rhodamine retention in the cells. After 30 h there was an increase in the number of cells cultured in 0.5 μM zinc medium that bound annexin V-FITC. These data indicated that decreased intracellular zinc concentration preceded early markers of apoptosis, with alterations in mitochondrial transmembrane potential preceding the loss of polarity in the cell membrane.

Original languageEnglish (US)
Pages (from-to)161-172
Number of pages12
JournalApoptosis
Volume6
Issue number3
DOIs
StatePublished - 2001

Fingerprint

HL-60 Cells
Zinc
Apoptosis
Rhodamine 123
Rhodamines
Flow cytometry
Fluorophores
Fluorescein-5-isothiocyanate
Annexin A5
Fluorescence microscopy
Cell membranes
Fluorescence Microscopy
Membrane Potentials
Cultured Cells
Flow Cytometry
Cell Count
Cells
Cell Membrane

Keywords

  • Annexin V
  • Apoptosis
  • Caspase-3
  • Cell death
  • Rhodamine 123
  • Zinc

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Duffy, J. Y., Miller, C. M., Rutschilling, G. L., Ridder, G. M., Clegg, M. S., Keen, C. L., & Daston, G. P. (2001). A decrease in intracellular zinc level precedes the detection of early indicators of apoptosis in HL-60 cells. Apoptosis, 6(3), 161-172. https://doi.org/10.1023/A:1011380508902

A decrease in intracellular zinc level precedes the detection of early indicators of apoptosis in HL-60 cells. / Duffy, J. Y.; Miller, C. M.; Rutschilling, G. L.; Ridder, G. M.; Clegg, M. S.; Keen, Carl L; Daston, G. P.

In: Apoptosis, Vol. 6, No. 3, 2001, p. 161-172.

Research output: Contribution to journalArticle

Duffy, JY, Miller, CM, Rutschilling, GL, Ridder, GM, Clegg, MS, Keen, CL & Daston, GP 2001, 'A decrease in intracellular zinc level precedes the detection of early indicators of apoptosis in HL-60 cells', Apoptosis, vol. 6, no. 3, pp. 161-172. https://doi.org/10.1023/A:1011380508902
Duffy, J. Y. ; Miller, C. M. ; Rutschilling, G. L. ; Ridder, G. M. ; Clegg, M. S. ; Keen, Carl L ; Daston, G. P. / A decrease in intracellular zinc level precedes the detection of early indicators of apoptosis in HL-60 cells. In: Apoptosis. 2001 ; Vol. 6, No. 3. pp. 161-172.
@article{5eab66bcfde74beab5658fd0325955dc,
title = "A decrease in intracellular zinc level precedes the detection of early indicators of apoptosis in HL-60 cells",
abstract = "Low extracellular zinc concentrations have been associated with the induction of apoptosis. To assess the relationship between intracellular zinc concentration and the rate of apoptosis, cells were grown in media containing 0.5, 25, or 50 μM zinc and analyzed by flow cytometry or fluorescence microscopy. Cells grown in 0.5 μM zinc medium over 48 h showed a successive decrease in intracellular zinc concentration measured by the zinc-specific fluorophore, zinquin. After 18 h in 0.5 μM zinc medium, rhodamine 123 retention decreased. However, the addition of 10 μM zinc to the 0.5 μM medium before 16 h in culture restored rhodamine retention in the cells. After 30 h there was an increase in the number of cells cultured in 0.5 μM zinc medium that bound annexin V-FITC. These data indicated that decreased intracellular zinc concentration preceded early markers of apoptosis, with alterations in mitochondrial transmembrane potential preceding the loss of polarity in the cell membrane.",
keywords = "Annexin V, Apoptosis, Caspase-3, Cell death, Rhodamine 123, Zinc",
author = "Duffy, {J. Y.} and Miller, {C. M.} and Rutschilling, {G. L.} and Ridder, {G. M.} and Clegg, {M. S.} and Keen, {Carl L} and Daston, {G. P.}",
year = "2001",
doi = "10.1023/A:1011380508902",
language = "English (US)",
volume = "6",
pages = "161--172",
journal = "Apoptosis : an international journal on programmed cell death",
issn = "1360-8185",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - A decrease in intracellular zinc level precedes the detection of early indicators of apoptosis in HL-60 cells

AU - Duffy, J. Y.

AU - Miller, C. M.

AU - Rutschilling, G. L.

AU - Ridder, G. M.

AU - Clegg, M. S.

AU - Keen, Carl L

AU - Daston, G. P.

PY - 2001

Y1 - 2001

N2 - Low extracellular zinc concentrations have been associated with the induction of apoptosis. To assess the relationship between intracellular zinc concentration and the rate of apoptosis, cells were grown in media containing 0.5, 25, or 50 μM zinc and analyzed by flow cytometry or fluorescence microscopy. Cells grown in 0.5 μM zinc medium over 48 h showed a successive decrease in intracellular zinc concentration measured by the zinc-specific fluorophore, zinquin. After 18 h in 0.5 μM zinc medium, rhodamine 123 retention decreased. However, the addition of 10 μM zinc to the 0.5 μM medium before 16 h in culture restored rhodamine retention in the cells. After 30 h there was an increase in the number of cells cultured in 0.5 μM zinc medium that bound annexin V-FITC. These data indicated that decreased intracellular zinc concentration preceded early markers of apoptosis, with alterations in mitochondrial transmembrane potential preceding the loss of polarity in the cell membrane.

AB - Low extracellular zinc concentrations have been associated with the induction of apoptosis. To assess the relationship between intracellular zinc concentration and the rate of apoptosis, cells were grown in media containing 0.5, 25, or 50 μM zinc and analyzed by flow cytometry or fluorescence microscopy. Cells grown in 0.5 μM zinc medium over 48 h showed a successive decrease in intracellular zinc concentration measured by the zinc-specific fluorophore, zinquin. After 18 h in 0.5 μM zinc medium, rhodamine 123 retention decreased. However, the addition of 10 μM zinc to the 0.5 μM medium before 16 h in culture restored rhodamine retention in the cells. After 30 h there was an increase in the number of cells cultured in 0.5 μM zinc medium that bound annexin V-FITC. These data indicated that decreased intracellular zinc concentration preceded early markers of apoptosis, with alterations in mitochondrial transmembrane potential preceding the loss of polarity in the cell membrane.

KW - Annexin V

KW - Apoptosis

KW - Caspase-3

KW - Cell death

KW - Rhodamine 123

KW - Zinc

UR - http://www.scopus.com/inward/record.url?scp=0035025062&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035025062&partnerID=8YFLogxK

U2 - 10.1023/A:1011380508902

DO - 10.1023/A:1011380508902

M3 - Article

C2 - 11388665

AN - SCOPUS:0035025062

VL - 6

SP - 161

EP - 172

JO - Apoptosis : an international journal on programmed cell death

JF - Apoptosis : an international journal on programmed cell death

SN - 1360-8185

IS - 3

ER -