A decrease in intracellular zinc level precedes the detection of early indicators of apoptosis in HL-60 cells

J. Y. Duffy, C. M. Miller, G. L. Rutschilling, G. M. Ridder, M. S. Clegg, Carl L Keen, G. P. Daston

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Low extracellular zinc concentrations have been associated with the induction of apoptosis. To assess the relationship between intracellular zinc concentration and the rate of apoptosis, cells were grown in media containing 0.5, 25, or 50 μM zinc and analyzed by flow cytometry or fluorescence microscopy. Cells grown in 0.5 μM zinc medium over 48 h showed a successive decrease in intracellular zinc concentration measured by the zinc-specific fluorophore, zinquin. After 18 h in 0.5 μM zinc medium, rhodamine 123 retention decreased. However, the addition of 10 μM zinc to the 0.5 μM medium before 16 h in culture restored rhodamine retention in the cells. After 30 h there was an increase in the number of cells cultured in 0.5 μM zinc medium that bound annexin V-FITC. These data indicated that decreased intracellular zinc concentration preceded early markers of apoptosis, with alterations in mitochondrial transmembrane potential preceding the loss of polarity in the cell membrane.

Original languageEnglish (US)
Pages (from-to)161-172
Number of pages12
JournalApoptosis
Volume6
Issue number3
DOIs
StatePublished - 2001

Keywords

  • Annexin V
  • Apoptosis
  • Caspase-3
  • Cell death
  • Rhodamine 123
  • Zinc

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

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    Duffy, J. Y., Miller, C. M., Rutschilling, G. L., Ridder, G. M., Clegg, M. S., Keen, C. L., & Daston, G. P. (2001). A decrease in intracellular zinc level precedes the detection of early indicators of apoptosis in HL-60 cells. Apoptosis, 6(3), 161-172. https://doi.org/10.1023/A:1011380508902