A cytoplasmic peptide of the neurotrophin receptor p75NTR

Induction of apoptosis and NMR determined helical conformation

Matthew R. Hileman, Barbara S. Chapman, S. Rabizadeh, Viswanathan V Krishnan, Dale Bredesen, Nuria Assa-Munt, Leigh A. Plesniak

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The neurotrophin receptor (NTR) and tumor necrosis factor receptor family of receptors regulate apoptotic cell death during development and in adult tissues [Beutler and van Huffel, Science 264 (1994) 667-668]. We have examined a fragment of p75NTR from the carboxyl terminus of the receptor and a variant form of this peptide via NMR techniques and in vitro assays for apoptotic activity. The wild type peptide induces apoptosis and adopts a helical conformation oriented parallel to the surface of lipid micelles, whereas the variant form adopts a non-helical conformation in the presence of lipid and shows no activity. These experiments suggest a link between structure and function of the two peptides.

Original languageEnglish (US)
Pages (from-to)145-154
Number of pages10
JournalFEBS Letters
Volume415
Issue number2
DOIs
StatePublished - Sep 29 1997
Externally publishedYes

Fingerprint

Nerve Growth Factor Receptors
Peptide Receptors
Conformations
Nuclear magnetic resonance
Apoptosis
Peptides
Lipids
Tumor Necrosis Factor Receptors
Micelles
Cell death
Assays
Cell Death
Tissue
Experiments

Keywords

  • Chemical shift index
  • Micelle
  • Spin label

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A cytoplasmic peptide of the neurotrophin receptor p75NTR : Induction of apoptosis and NMR determined helical conformation. / Hileman, Matthew R.; Chapman, Barbara S.; Rabizadeh, S.; Krishnan, Viswanathan V; Bredesen, Dale; Assa-Munt, Nuria; Plesniak, Leigh A.

In: FEBS Letters, Vol. 415, No. 2, 29.09.1997, p. 145-154.

Research output: Contribution to journalArticle

Hileman, Matthew R. ; Chapman, Barbara S. ; Rabizadeh, S. ; Krishnan, Viswanathan V ; Bredesen, Dale ; Assa-Munt, Nuria ; Plesniak, Leigh A. / A cytoplasmic peptide of the neurotrophin receptor p75NTR : Induction of apoptosis and NMR determined helical conformation. In: FEBS Letters. 1997 ; Vol. 415, No. 2. pp. 145-154.
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