TY - JOUR
T1 - A cyclin A-protein kinase complex possesses sequence-specific DNA binding activity
T2 - p33cdk2 is a component of the E2F-cyclin A complex
AU - Devoto, Stephen H.
AU - Mudryj, Maria
AU - Pines, Jonathon
AU - Hunter, Tony
AU - Nevins, Joseph R.
PY - 1992/1/10
Y1 - 1992/1/10
N2 - The E2F transcription factor has been found in association with the cyclin A protein, and this complex accumulates during the S phase of the cell cycle, suggesting that E2F may play a role in cell cycle control. In independent studies, cyclin A has been shown to be associated with two other proteins, the Rb-related p107 protein and the cdc2-related p33 cdk2 protein kinase. Through an analysis of the E2F-cyclin A complex, we now find that both the p107 protein and the cdc2-related p33cdk2 kinase are components of the previously described complex. Moreover, the complex possesses H1 kinase activity. These results thus define a cyclin A-cdk2 kinase complex that possesses sequence-specific DNA binding activity. This suggests that the cdk2 kinase may phosphorylate other DNA-bound substrates, and that one role of the E2F factor may be to localize this protein kinase to the DNA.
AB - The E2F transcription factor has been found in association with the cyclin A protein, and this complex accumulates during the S phase of the cell cycle, suggesting that E2F may play a role in cell cycle control. In independent studies, cyclin A has been shown to be associated with two other proteins, the Rb-related p107 protein and the cdc2-related p33 cdk2 protein kinase. Through an analysis of the E2F-cyclin A complex, we now find that both the p107 protein and the cdc2-related p33cdk2 kinase are components of the previously described complex. Moreover, the complex possesses H1 kinase activity. These results thus define a cyclin A-cdk2 kinase complex that possesses sequence-specific DNA binding activity. This suggests that the cdk2 kinase may phosphorylate other DNA-bound substrates, and that one role of the E2F factor may be to localize this protein kinase to the DNA.
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U2 - 10.1016/0092-8674(92)90215-X
DO - 10.1016/0092-8674(92)90215-X
M3 - Article
C2 - 1310073
AN - SCOPUS:0026536876
VL - 68
SP - 167
EP - 176
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -