A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo

Hatim A. Zariwala, Bart G. Borghuis, Tycho M. Hoogland, Linda Madisen, Lin Tian, Chris I. de Zeeuw, Hongkui Zeng, Loren L. Looger, Karel Svoboda, Tsai Wen Chen

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

Fluorescent calcium indicator proteins, such as GCaMP3, allow imaging of activity in genetically defined neuronal populations. GCaMP3 can be expressed using various gene delivery methods, such as viral infection or electroporation. However, these methods are invasive and provide inhomogeneous and nonstationary expression. Here, we developed a genetic reporter mouse, Ai38, which expressesGCaMP3 in a Cre-dependent manner from the ROSA26 locus, driven by a strong CAG promoter. Crossing Ai38 with appropriate Cre mice produced robust GCaMP3 expression in defined cell populations in the retina, cortex, and cerebellum. In the primary visual cortex, visually evoked GCaMP3 signals showed normal orientation and direction selectivity. GCaMP3 signals were rapid, compared with virally expressed GCaMP3 and synthetic calcium indicators. In the retina, Ai38 allowed imaging spontaneous calcium waves in starburst amacrine cells during development, and light-evoked responses in ganglion cells in adult tissue. Our results show that the Ai38 reporter mouse provides a flexible method for targeted expression of GCaMP3.

Original languageEnglish (US)
Pages (from-to)3131-3141
Number of pages11
JournalJournal of Neuroscience
Volume32
Issue number9
DOIs
StatePublished - Feb 29 2012
Externally publishedYes

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Retina
Calcium
Amacrine Cells
Electroporation
Calcium Signaling
Visual Cortex
Virus Diseases
Ganglia
Cerebellum
Population
Light
Genes
Proteins
Direction compound

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Zariwala, H. A., Borghuis, B. G., Hoogland, T. M., Madisen, L., Tian, L., de Zeeuw, C. I., ... Chen, T. W. (2012). A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo. Journal of Neuroscience, 32(9), 3131-3141. https://doi.org/10.1523/JNEUROSCI.4469-11.2012

A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo. / Zariwala, Hatim A.; Borghuis, Bart G.; Hoogland, Tycho M.; Madisen, Linda; Tian, Lin; de Zeeuw, Chris I.; Zeng, Hongkui; Looger, Loren L.; Svoboda, Karel; Chen, Tsai Wen.

In: Journal of Neuroscience, Vol. 32, No. 9, 29.02.2012, p. 3131-3141.

Research output: Contribution to journalArticle

Zariwala, HA, Borghuis, BG, Hoogland, TM, Madisen, L, Tian, L, de Zeeuw, CI, Zeng, H, Looger, LL, Svoboda, K & Chen, TW 2012, 'A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo', Journal of Neuroscience, vol. 32, no. 9, pp. 3131-3141. https://doi.org/10.1523/JNEUROSCI.4469-11.2012
Zariwala HA, Borghuis BG, Hoogland TM, Madisen L, Tian L, de Zeeuw CI et al. A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo. Journal of Neuroscience. 2012 Feb 29;32(9):3131-3141. https://doi.org/10.1523/JNEUROSCI.4469-11.2012
Zariwala, Hatim A. ; Borghuis, Bart G. ; Hoogland, Tycho M. ; Madisen, Linda ; Tian, Lin ; de Zeeuw, Chris I. ; Zeng, Hongkui ; Looger, Loren L. ; Svoboda, Karel ; Chen, Tsai Wen. / A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 9. pp. 3131-3141.
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