A correlation of Salmonella mutagenicity with DNA adducts induced by the cooked-food mutagen 2-amino-1-methyl-6-6-phenylimidazo[4,5,-b]pyridine

Michael A. Malfatti, Nancy H. Shen, Rebekah W. Wu, Ken W Turteltaub, James S. Felton

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The correlation of bacterial mutagenicity with DNA adducts from the heterocyclic amine cooked-food mutagen 2-amino-l-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) was investigated in Salmonella typhimurium strains TA98 (uvrB deficient) and TA1978 (uvrB proficient). Bacterial cells were exposed to PhIP using a modification of the Ames/ Salmonella microsuspension assay. Half of the cells, generated from a 90 min pre-incubation and washing, were plated for revertant formation while the remaining half was subjected to DNA adduct analysis via 32P-postlabeling. In TA98, DNA adducts were detected at an RAL (relative adduct labeling) of 10×10-7 and 21×10-7 at PhIP concentrations of 5.5 and 17 μM, respectively. This corresponded to 28.8 and 20.9 adducts/revertant, respectively. These values were based on the assumption that only four repeating GC bases within a 75 DNA base region is the gene target site for PhIP induced mutations. In TA1978, no revertants above background were detected at any concentration of PhIP tested. DNA adducts, however, were detected at 11×10-7 and 21×10-7 adducts per nucleotide at 223 and 1116 μM PhIP, respectively. The lack of detectable revertants, but the presence of DNA adducts, suggests pre-mutational lesions did occur during the 90 min pre-incubation. Presumably, when the S9 activating system and PhIP were removed (via washing with phosphate buffered saline) prior to plating, the cells containing an intact uvrB repair system repaired the lesions during the incubation time on the plates. In conclusion, the induction of revertants by adducts appears quite efficient, as ∼25 adducts are required for one mutational event in the excision repair deficient bacteria.

Original languageEnglish (US)
Pages (from-to)425-431
Number of pages7
JournalMutagenesis
Volume10
Issue number5
DOIs
StatePublished - Sep 1995
Externally publishedYes

Fingerprint

Mutagens
Salmonella
DNA Adducts
Pyridine
Food
Repair
Cell
Washing
Phosphate
Bacteria
Labeling
Proof by induction
Mutation
Gene
Nucleotides
Target
Plating
Amines
Assays
Phosphates

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

A correlation of Salmonella mutagenicity with DNA adducts induced by the cooked-food mutagen 2-amino-1-methyl-6-6-phenylimidazo[4,5,-b]pyridine. / Malfatti, Michael A.; Shen, Nancy H.; Wu, Rebekah W.; Turteltaub, Ken W; Felton, James S.

In: Mutagenesis, Vol. 10, No. 5, 09.1995, p. 425-431.

Research output: Contribution to journalArticle

Malfatti, Michael A. ; Shen, Nancy H. ; Wu, Rebekah W. ; Turteltaub, Ken W ; Felton, James S. / A correlation of Salmonella mutagenicity with DNA adducts induced by the cooked-food mutagen 2-amino-1-methyl-6-6-phenylimidazo[4,5,-b]pyridine. In: Mutagenesis. 1995 ; Vol. 10, No. 5. pp. 425-431.
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abstract = "The correlation of bacterial mutagenicity with DNA adducts from the heterocyclic amine cooked-food mutagen 2-amino-l-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) was investigated in Salmonella typhimurium strains TA98 (uvrB deficient) and TA1978 (uvrB proficient). Bacterial cells were exposed to PhIP using a modification of the Ames/ Salmonella microsuspension assay. Half of the cells, generated from a 90 min pre-incubation and washing, were plated for revertant formation while the remaining half was subjected to DNA adduct analysis via 32P-postlabeling. In TA98, DNA adducts were detected at an RAL (relative adduct labeling) of 10×10-7 and 21×10-7 at PhIP concentrations of 5.5 and 17 μM, respectively. This corresponded to 28.8 and 20.9 adducts/revertant, respectively. These values were based on the assumption that only four repeating GC bases within a 75 DNA base region is the gene target site for PhIP induced mutations. In TA1978, no revertants above background were detected at any concentration of PhIP tested. DNA adducts, however, were detected at 11×10-7 and 21×10-7 adducts per nucleotide at 223 and 1116 μM PhIP, respectively. The lack of detectable revertants, but the presence of DNA adducts, suggests pre-mutational lesions did occur during the 90 min pre-incubation. Presumably, when the S9 activating system and PhIP were removed (via washing with phosphate buffered saline) prior to plating, the cells containing an intact uvrB repair system repaired the lesions during the incubation time on the plates. In conclusion, the induction of revertants by adducts appears quite efficient, as ∼25 adducts are required for one mutational event in the excision repair deficient bacteria.",
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