A convenient method for evaluating epithelial cell proliferation in the whole mammary glands of female mice

Grace E. Berryhill, Ingrid Brust-Mascher, Jill H. Huynh, Thomas R. Famula, Colin Reardon, Russell C. Hovey

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The mammary glands (MG) undergo rapid expansion of the ductal network during puberty in response to endocrine cues including the potent mitogenic effects of estrogen. The proliferation of mammary epithelial cells occurs in a spatially distinctive manner, where terminal end buds located at the ductal termini are the primary site of cell division. Here, we present a relatively high throughput approach to spatially assess epithelial cell proliferation in whole mouse MG using histochemical detection of 5-ethynyl-2'-deoxyuridine in conjunction with a standard curve-based data deconvolution technique to semiquantitatively measure proliferation via wide-field epifluorescent microscopy. This approach was validated against the "gold standard" of counting labeled nuclei from confocal images utilizing computer-assisted image analysis. Our method proved sensitive enough to describe the significant and spatially variable proliferative response to low-dose estrogen after 108 hours. This flexible method presents a timely and economical approach to obtaining spatial information regarding epithelial cell proliferation in the mouse MG.

Original languageEnglish (US)
Pages (from-to)3742-3748
Number of pages7
JournalEndocrinology
Volume157
Issue number10
DOIs
StatePublished - 2016

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Human Mammary Glands
Epithelial Cells
Cell Proliferation
Estrogens
Computer-Assisted Image Processing
Puberty
Cell Division
Cues
Microscopy
Breast

ASJC Scopus subject areas

  • Endocrinology

Cite this

Berryhill, G. E., Brust-Mascher, I., Huynh, J. H., Famula, T. R., Reardon, C., & Hovey, R. C. (2016). A convenient method for evaluating epithelial cell proliferation in the whole mammary glands of female mice. Endocrinology, 157(10), 3742-3748. https://doi.org/10.1210/en.2016-1480

A convenient method for evaluating epithelial cell proliferation in the whole mammary glands of female mice. / Berryhill, Grace E.; Brust-Mascher, Ingrid; Huynh, Jill H.; Famula, Thomas R.; Reardon, Colin; Hovey, Russell C.

In: Endocrinology, Vol. 157, No. 10, 2016, p. 3742-3748.

Research output: Contribution to journalArticle

Berryhill, GE, Brust-Mascher, I, Huynh, JH, Famula, TR, Reardon, C & Hovey, RC 2016, 'A convenient method for evaluating epithelial cell proliferation in the whole mammary glands of female mice', Endocrinology, vol. 157, no. 10, pp. 3742-3748. https://doi.org/10.1210/en.2016-1480
Berryhill, Grace E. ; Brust-Mascher, Ingrid ; Huynh, Jill H. ; Famula, Thomas R. ; Reardon, Colin ; Hovey, Russell C. / A convenient method for evaluating epithelial cell proliferation in the whole mammary glands of female mice. In: Endocrinology. 2016 ; Vol. 157, No. 10. pp. 3742-3748.
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