Abstract
Dynamin-related proteins (DRPs) compose a diverse family of proteins that function, through GTPase stimulated self-assembly, to remodel cellular membranes. The molecular mechanism by which DRPs mediate membrane remodeling events and the specific role of their GTPase cycle is still not fully understood. Although DRPs are members of the GTPase superfamily, they possess unique kinetic properties. In particular, they have relatively low affinity for guanine nucleotides and, under conditions that favor self-assembly, they have high rates of GTP turnover. Established fixed time point assays used for the analysis of assembly stimulated GTPase activity are prone to inaccuracies due to substrate depletion and are also limited by lack of time resolution. We describe a simple, continuous, coupled GTP regenerating assay that tackles the limitations of the fixed time point assays and can be used for the kinetic analysis of DRP GTP hydrolysis under unassembled and assembled conditions.
| Original language | English (US) |
|---|---|
| Article number | 53 |
| Pages (from-to) | 611-619 |
| Number of pages | 9 |
| Journal | Methods in Enzymology |
| Volume | 404 |
| DOIs | |
| State | Published - 2006 |
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ASJC Scopus subject areas
- Biochemistry
Cite this
A continuous, regenerative coupled GTPase assay for dynamin-related proteins. / Ingerman, Elena; Nunnari, Jodi.
In: Methods in Enzymology, Vol. 404, 53, 2006, p. 611-619.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A continuous, regenerative coupled GTPase assay for dynamin-related proteins
AU - Ingerman, Elena
AU - Nunnari, Jodi
PY - 2006
Y1 - 2006
N2 - Dynamin-related proteins (DRPs) compose a diverse family of proteins that function, through GTPase stimulated self-assembly, to remodel cellular membranes. The molecular mechanism by which DRPs mediate membrane remodeling events and the specific role of their GTPase cycle is still not fully understood. Although DRPs are members of the GTPase superfamily, they possess unique kinetic properties. In particular, they have relatively low affinity for guanine nucleotides and, under conditions that favor self-assembly, they have high rates of GTP turnover. Established fixed time point assays used for the analysis of assembly stimulated GTPase activity are prone to inaccuracies due to substrate depletion and are also limited by lack of time resolution. We describe a simple, continuous, coupled GTP regenerating assay that tackles the limitations of the fixed time point assays and can be used for the kinetic analysis of DRP GTP hydrolysis under unassembled and assembled conditions.
AB - Dynamin-related proteins (DRPs) compose a diverse family of proteins that function, through GTPase stimulated self-assembly, to remodel cellular membranes. The molecular mechanism by which DRPs mediate membrane remodeling events and the specific role of their GTPase cycle is still not fully understood. Although DRPs are members of the GTPase superfamily, they possess unique kinetic properties. In particular, they have relatively low affinity for guanine nucleotides and, under conditions that favor self-assembly, they have high rates of GTP turnover. Established fixed time point assays used for the analysis of assembly stimulated GTPase activity are prone to inaccuracies due to substrate depletion and are also limited by lack of time resolution. We describe a simple, continuous, coupled GTP regenerating assay that tackles the limitations of the fixed time point assays and can be used for the kinetic analysis of DRP GTP hydrolysis under unassembled and assembled conditions.
UR - http://www.scopus.com/inward/record.url?scp=30544450009&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=30544450009&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(05)04053-X
DO - 10.1016/S0076-6879(05)04053-X
M3 - Article
C2 - 16413304
AN - SCOPUS:30544450009
VL - 404
SP - 611
EP - 619
JO - Methods
JF - Methods
SN - 0076-6879
M1 - 53
ER -