A complex glutathione transferase gene family in the housefly Musca domestica

Z. H. Zhou, Michael Syvanen

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

In most metazoans, the glutathione S-transferases (GST) are encoded by gene families, and are used to detoxify xenobiotics. We describe the structure of genomic loci coding for the GSTs in the housefly that have been implicated, by both genetic and biochemical means, in mediating insecticide resistance. In earlier work, we showed that one of the theta-class enzymes, MdGST-3, is overproduced in resistant flies and degrades certain insecticides. We used a fragment from a cDNA clone of MdGST-3 as a probe to screen a housefly genomic DNA bank in phage λ. This probe detected multiple gst loci. Genes for GSTs were found in five different, nonoverlapping λ, clones, three of which carry multiple, closely linked gsts. Multiple genes for both MdGST-3 and MdGST-4 were found; some of which have introns in their 5' untranslated regions. In adults, the only MdGST-3 enzymes that are expressed are encoded by the intron-free genes. A new theta-class GST (called MdGST-5) was also discovered. Fusion genes comprising 5' MdGST-3 sequences and either MdGST-4 or MdGST-5 sequences in their 3' halves were encountered at three separate loci. The genes described here are found in both the ancestral sensitive strain and the insecticide-resistant strains.

Original languageEnglish (US)
Pages (from-to)187-194
Number of pages8
JournalMolecular and General Genetics
Volume256
Issue number2
DOIs
StatePublished - 1997

Fingerprint

Houseflies
Glutathione Transferase
Genes
Insecticides
Introns
Clone Cells
Insecticide Resistance
5' Untranslated Regions
Gene Fusion
Xenobiotics
Enzymes
Diptera
Bacteriophages
Molecular Biology
Complementary DNA
DNA

Keywords

  • Gene fusion
  • Glutathione S-transferase
  • Housefly
  • Multigene family
  • Organophosphate insecticide

ASJC Scopus subject areas

  • Genetics

Cite this

A complex glutathione transferase gene family in the housefly Musca domestica. / Zhou, Z. H.; Syvanen, Michael.

In: Molecular and General Genetics, Vol. 256, No. 2, 1997, p. 187-194.

Research output: Contribution to journalArticle

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