A competitive enzyme-linked immunosorbent assay (ELISA) to detect retronecine and monocrotaline in vitro

Mary A. Bober; Larry A. Milco; R. Bryan Miller; Michael Mount; Berry Wicks; Mark J. Kurth

doi:10.1016/0041-0101(89)90158-X

A competitive enzyme-linked immunosorbent assay (ELISA) to detect retronecine and monocrotaline in vitro

Mary A. Bober, Larry A. Milco, R. Bryan Miller, Michael Mount, Berry Wicks, Mark J. Kurth

Chemistry

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Antibodies to the nonesterified pyrrolizidine nucleus, retronecine (155 mol.wt), were produced in rabbits and detected using an avidin-biotin antibody ELISA. A competitive ELISA for the detection of retronecine and the cyclic diester monocrotaline was also developed using the antiserum produced against the hapten conjugate, retronecine-bovine serum albumin. Retronecine was obtained by hydrolysis of monocrotaline, succinylated and directly coupled to bovine serum albumin or ovalbumin. Antibodies to the pyrrolizidine nucleus, retronecine, can be detected within 5 min after the addition of substrate using the avidin-biotin ELISA. Competitive inhibition of antibodies to retronecine is obtained by the addition of known amounts (0-11.42 μg/μl) of either the homologous antigen, retronecine, or the heterologous antigen, monocrotaline, however, retronecine acts as the better competitor.

Original language	English (US)
Pages (from-to)	1059-1064
Number of pages	6
Journal	Toxicon
Volume	27
Issue number	9
DOIs	https://doi.org/10.1016/0041-0101(89)90158-X
State	Published - 1989

ASJC Scopus subject areas

Toxicology

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title = "A competitive enzyme-linked immunosorbent assay (ELISA) to detect retronecine and monocrotaline in vitro",

abstract = "Antibodies to the nonesterified pyrrolizidine nucleus, retronecine (155 mol.wt), were produced in rabbits and detected using an avidin-biotin antibody ELISA. A competitive ELISA for the detection of retronecine and the cyclic diester monocrotaline was also developed using the antiserum produced against the hapten conjugate, retronecine-bovine serum albumin. Retronecine was obtained by hydrolysis of monocrotaline, succinylated and directly coupled to bovine serum albumin or ovalbumin. Antibodies to the pyrrolizidine nucleus, retronecine, can be detected within 5 min after the addition of substrate using the avidin-biotin ELISA. Competitive inhibition of antibodies to retronecine is obtained by the addition of known amounts (0-11.42 μg/μl) of either the homologous antigen, retronecine, or the heterologous antigen, monocrotaline, however, retronecine acts as the better competitor.",

author = "Bober, {Mary A.} and Milco, {Larry A.} and Miller, {R. Bryan} and Michael Mount and Berry Wicks and Kurth, {Mark J.}",

year = "1989",

doi = "10.1016/0041-0101(89)90158-X",

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T1 - A competitive enzyme-linked immunosorbent assay (ELISA) to detect retronecine and monocrotaline in vitro

AU - Bober, Mary A.

AU - Milco, Larry A.

AU - Miller, R. Bryan

AU - Mount, Michael

AU - Wicks, Berry

AU - Kurth, Mark J.

PY - 1989

Y1 - 1989

N2 - Antibodies to the nonesterified pyrrolizidine nucleus, retronecine (155 mol.wt), were produced in rabbits and detected using an avidin-biotin antibody ELISA. A competitive ELISA for the detection of retronecine and the cyclic diester monocrotaline was also developed using the antiserum produced against the hapten conjugate, retronecine-bovine serum albumin. Retronecine was obtained by hydrolysis of monocrotaline, succinylated and directly coupled to bovine serum albumin or ovalbumin. Antibodies to the pyrrolizidine nucleus, retronecine, can be detected within 5 min after the addition of substrate using the avidin-biotin ELISA. Competitive inhibition of antibodies to retronecine is obtained by the addition of known amounts (0-11.42 μg/μl) of either the homologous antigen, retronecine, or the heterologous antigen, monocrotaline, however, retronecine acts as the better competitor.

AB - Antibodies to the nonesterified pyrrolizidine nucleus, retronecine (155 mol.wt), were produced in rabbits and detected using an avidin-biotin antibody ELISA. A competitive ELISA for the detection of retronecine and the cyclic diester monocrotaline was also developed using the antiserum produced against the hapten conjugate, retronecine-bovine serum albumin. Retronecine was obtained by hydrolysis of monocrotaline, succinylated and directly coupled to bovine serum albumin or ovalbumin. Antibodies to the pyrrolizidine nucleus, retronecine, can be detected within 5 min after the addition of substrate using the avidin-biotin ELISA. Competitive inhibition of antibodies to retronecine is obtained by the addition of known amounts (0-11.42 μg/μl) of either the homologous antigen, retronecine, or the heterologous antigen, monocrotaline, however, retronecine acts as the better competitor.

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