A competitive enzyme-linked immunosorbent assay (ELISA) to detect retronecine and monocrotaline in vitro

Mary A. Bober, Larry A. Milco, R. Bryan Miller, Michael Mount, Berry Wicks, Mark J. Kurth

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Antibodies to the nonesterified pyrrolizidine nucleus, retronecine (155 mol.wt), were produced in rabbits and detected using an avidin-biotin antibody ELISA. A competitive ELISA for the detection of retronecine and the cyclic diester monocrotaline was also developed using the antiserum produced against the hapten conjugate, retronecine-bovine serum albumin. Retronecine was obtained by hydrolysis of monocrotaline, succinylated and directly coupled to bovine serum albumin or ovalbumin. Antibodies to the pyrrolizidine nucleus, retronecine, can be detected within 5 min after the addition of substrate using the avidin-biotin ELISA. Competitive inhibition of antibodies to retronecine is obtained by the addition of known amounts (0-11.42 μg/μl) of either the homologous antigen, retronecine, or the heterologous antigen, monocrotaline, however, retronecine acts as the better competitor.

Original languageEnglish (US)
Pages (from-to)1059-1064
Number of pages6
JournalToxicon
Volume27
Issue number9
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Toxicology

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    Bober, M. A., Milco, L. A., Miller, R. B., Mount, M., Wicks, B., & Kurth, M. J. (1989). A competitive enzyme-linked immunosorbent assay (ELISA) to detect retronecine and monocrotaline in vitro. Toxicon, 27(9), 1059-1064. https://doi.org/10.1016/0041-0101(89)90158-X