TY - JOUR
T1 - A competitive enzyme-linked immunosorbent assay (ELISA) to detect retronecine and monocrotaline in vitro
AU - Bober, Mary A.
AU - Milco, Larry A.
AU - Miller, R. Bryan
AU - Mount, Michael
AU - Wicks, Berry
AU - Kurth, Mark J.
PY - 1989
Y1 - 1989
N2 - Antibodies to the nonesterified pyrrolizidine nucleus, retronecine (155 mol.wt), were produced in rabbits and detected using an avidin-biotin antibody ELISA. A competitive ELISA for the detection of retronecine and the cyclic diester monocrotaline was also developed using the antiserum produced against the hapten conjugate, retronecine-bovine serum albumin. Retronecine was obtained by hydrolysis of monocrotaline, succinylated and directly coupled to bovine serum albumin or ovalbumin. Antibodies to the pyrrolizidine nucleus, retronecine, can be detected within 5 min after the addition of substrate using the avidin-biotin ELISA. Competitive inhibition of antibodies to retronecine is obtained by the addition of known amounts (0-11.42 μg/μl) of either the homologous antigen, retronecine, or the heterologous antigen, monocrotaline, however, retronecine acts as the better competitor.
AB - Antibodies to the nonesterified pyrrolizidine nucleus, retronecine (155 mol.wt), were produced in rabbits and detected using an avidin-biotin antibody ELISA. A competitive ELISA for the detection of retronecine and the cyclic diester monocrotaline was also developed using the antiserum produced against the hapten conjugate, retronecine-bovine serum albumin. Retronecine was obtained by hydrolysis of monocrotaline, succinylated and directly coupled to bovine serum albumin or ovalbumin. Antibodies to the pyrrolizidine nucleus, retronecine, can be detected within 5 min after the addition of substrate using the avidin-biotin ELISA. Competitive inhibition of antibodies to retronecine is obtained by the addition of known amounts (0-11.42 μg/μl) of either the homologous antigen, retronecine, or the heterologous antigen, monocrotaline, however, retronecine acts as the better competitor.
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U2 - 10.1016/0041-0101(89)90158-X
DO - 10.1016/0041-0101(89)90158-X
M3 - Article
C2 - 2508272
AN - SCOPUS:0024441093
VL - 27
SP - 1059
EP - 1064
JO - Toxicon
JF - Toxicon
SN - 0041-0101
IS - 9
ER -