A comparison of two probiotic strains of bifidobacteria in premature infants

Mark Underwood, Karen M. Kalanetra, Nicholas A. Bokulich, Zachery T. Lewis, Majid Mirmiran, Daniel J Tancredi, David A. Mills

Research output: Contribution to journalArticle

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Abstract

Objective To determine the impact of 2 probiotic bifidobacteria on the fecal microbiota of premature infants fed either human milk or formula. Study design In the first of two phase 1 clinical trials, 12 premature infants receiving formula feedings were assigned randomly to receive either Bifidobacterium longum ssp infantis or Bifidobacterium animalis ssp lactis in increasing doses during a 5-week period. In the second, 9 premature infants receiving their mother's milk received each of the two bifidobacteria for 2 weeks separated by a 1-week washout period. Serial stool specimens from each infant were analyzed by terminal restriction fragment-length polymorphism and quantitative polymerase chain reaction for bacterial composition. Results Among the formula-fed infants, there was a greater increase in fecal bifidobacteria among infants receiving B infantis (Binf) than those receiving B lactis (Blac). This difference was most marked at a dose of 1.4 × 109 colony-forming units twice daily (P <.05). Bacterial diversity improved over dose/time in those infants receiving Binf. Among the human milk-fed infants, greater increases in fecal bifidobacteria and decreases in γ- Proteobacteria followed the administration of Binf than Blac. The B longum group (which includes Binf but not Blac) was the dominant bifidobacteria among the human milk-fed infants, regardless of the probiotic administered. Conclusions Binf was more effective at colonizing the fecal microbiota than Blac in both formula-fed and human milk-fed premature infants. The combination of human milk plus Binf resulted in the greatest fecal levels of bifidobacteria.

Original languageEnglish (US)
JournalJournal of Pediatrics
Volume163
Issue number6
DOIs
StatePublished - Dec 2013

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Bifidobacterium
Probiotics
Premature Infants
Human Milk
Infant Formula
Microbiota
Sulfalene
Proteobacteria
Clinical Trials, Phase I
Restriction Fragment Length Polymorphisms
Milk
Stem Cells
Mothers
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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A comparison of two probiotic strains of bifidobacteria in premature infants. / Underwood, Mark; Kalanetra, Karen M.; Bokulich, Nicholas A.; Lewis, Zachery T.; Mirmiran, Majid; Tancredi, Daniel J; Mills, David A.

In: Journal of Pediatrics, Vol. 163, No. 6, 12.2013.

Research output: Contribution to journalArticle

Underwood, Mark ; Kalanetra, Karen M. ; Bokulich, Nicholas A. ; Lewis, Zachery T. ; Mirmiran, Majid ; Tancredi, Daniel J ; Mills, David A. / A comparison of two probiotic strains of bifidobacteria in premature infants. In: Journal of Pediatrics. 2013 ; Vol. 163, No. 6.
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AB - Objective To determine the impact of 2 probiotic bifidobacteria on the fecal microbiota of premature infants fed either human milk or formula. Study design In the first of two phase 1 clinical trials, 12 premature infants receiving formula feedings were assigned randomly to receive either Bifidobacterium longum ssp infantis or Bifidobacterium animalis ssp lactis in increasing doses during a 5-week period. In the second, 9 premature infants receiving their mother's milk received each of the two bifidobacteria for 2 weeks separated by a 1-week washout period. Serial stool specimens from each infant were analyzed by terminal restriction fragment-length polymorphism and quantitative polymerase chain reaction for bacterial composition. Results Among the formula-fed infants, there was a greater increase in fecal bifidobacteria among infants receiving B infantis (Binf) than those receiving B lactis (Blac). This difference was most marked at a dose of 1.4 × 109 colony-forming units twice daily (P <.05). Bacterial diversity improved over dose/time in those infants receiving Binf. Among the human milk-fed infants, greater increases in fecal bifidobacteria and decreases in γ- Proteobacteria followed the administration of Binf than Blac. The B longum group (which includes Binf but not Blac) was the dominant bifidobacteria among the human milk-fed infants, regardless of the probiotic administered. Conclusions Binf was more effective at colonizing the fecal microbiota than Blac in both formula-fed and human milk-fed premature infants. The combination of human milk plus Binf resulted in the greatest fecal levels of bifidobacteria.

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