A comparison of the anti-histone and Apop-Tag technique for demonstrating apoptosis with option for silver enhancement

Amal A. Al-Hazzaa, I. D. Bowen, M. A. Birchall

Research output: Contribution to journalArticle

7 Scopus citations


A novel immunocytochemical method is presented for the qualitative detection of DNA fragmentation in apoptosis. Anti-histone antibody is employed to localize exposed nucleosomal histones (H1, H2a, H2b, H3 and H4) rather than tagging the cut ends of fragmenting DNA as in conventional technique. The method was tested on squamous cell carcinoma of the larynx routinely fixed in formaldehyde and embedded in paraffin wax and compared with results obtained employing Apop-Tag kit (Oncor).

Original languageEnglish (US)
Pages (from-to)271-276
Number of pages6
JournalCell Biology International
Issue number4
StatePublished - Apr 1998
Externally publishedYes



  • Anti-histone
  • Apop-Tag
  • Apoptosis
  • Laryngeal carcinoma
  • Silver enhancement

ASJC Scopus subject areas

  • Cell Biology

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