A comparison of four apparatuses for recovering infectious pancreatic necrosis virus from rainbow trout

Ronald Hedrick, T. McDowell, R. Rosemark, D. Aronstein, L. Chan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Four tissue dispersion apparatuses were compared to determine which was most effective in recovering infectious pancreatic necrosis virus (IPNV) from rainbow trout (Salmo gairdneri). The Omnitron, Polytron, Stomacher, and mortar and pestle, routinely used in fish virology laboratories, were equally effective in liberating IPNV from carrier rainbow trout tissues. Viruses were found in 50-52.5% of the fish with each apparatus and there were no significant (P = 0.05) differences in the concentrations of virus recovered by the four methods. The results indicated that several apparatuses are suitable for recovering IPNV from trout tissues. The advantages and disadvantages of each piece of equipment are discussed.

Original languageEnglish (US)
Pages (from-to)47-51
Number of pages5
JournalProgressive Fish-Culturist
Volume48
Issue number1
DOIs
StatePublished - Jan 1 1986

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Infectious pancreatic necrosis virus
Oncorhynchus mykiss
rainbow
virus
Fishes
Viruses
virology
viruses
Virology
Trout
fish
trout
mortar
Equipment and Supplies
tissues
necrosis
comparison
tissue
methodology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

A comparison of four apparatuses for recovering infectious pancreatic necrosis virus from rainbow trout. / Hedrick, Ronald; McDowell, T.; Rosemark, R.; Aronstein, D.; Chan, L.

In: Progressive Fish-Culturist, Vol. 48, No. 1, 01.01.1986, p. 47-51.

Research output: Contribution to journalArticle

Hedrick, Ronald ; McDowell, T. ; Rosemark, R. ; Aronstein, D. ; Chan, L. / A comparison of four apparatuses for recovering infectious pancreatic necrosis virus from rainbow trout. In: Progressive Fish-Culturist. 1986 ; Vol. 48, No. 1. pp. 47-51.
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