Four tissue dispersion apparatuses were compared to determine which was most effective in recovering infectious pancreatic necrosis virus (IPNV) from rainbow trout (Salmo gairdneri). The Omnitron, Polytron, Stomacher, and mortar and pestle, routinely used in fish virology laboratories, were equally effective in liberating IPNV from carrier rainbow trout tissues. Viruses were found in 50-52.5% of the fish with each apparatus and there were no significant (P = 0.05) differences in the concentrations of virus recovered by the four methods. The results indicated that several apparatuses are suitable for recovering IPNV from trout tissues. The advantages and disadvantages of each piece of equipment are discussed.
|Original language||English (US)|
|Number of pages||5|
|State||Published - Jan 1 1986|
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)