A comparison of detergent action on supported lipid monolayers and bilayers

Viviane N. Ngassam, Michael C. Howland, Annapoorna Sapuri-Butti, Nathan Rosidi, Atul N. Parikh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Using spatially patterned supported lipid mono- and bilayers, we compare the effect of transleaflet dynamics on membrane solubilization by a common, non-ionic detergent in single samples. We find that at concentrations surrounding CMC, complete bilayers undergo 5-8% lateral expansion followed by rapid dissolution. In contrast, single supported monolayers remain remarkably resistant to solubilization, suggesting the central role of detergent or lipid flip-flop in driving membrane solubilization. In addition to the previously well-appreciated mode of detergent-resistance by tight lateral packing of saturated and cholesterol-rich lipids (e.g., rafts) in membrane bilayers, our results suggest that hindrance to interleaflet dynamics, such as by strong interaction with the cytoskeleton, provides an alternative mechanism by which membranes resist detergent solubilisation. Furthermore, we show that this differential resistance can be exploited to design spatial compositional patterns of lipid bilayers and monolayers.

Original languageEnglish (US)
Pages (from-to)3734-3738
Number of pages5
JournalSoft Matter
Volume8
Issue number14
DOIs
StatePublished - Apr 14 2012

Fingerprint

detergents
Detergents
lipids
Monolayers
membranes
Membranes
Lipids
rafts
flip-flops
Lipid bilayers
Flip flop circuits
cholesterol
dissolving
Dissolution
Cholesterol
expansion

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Ngassam, V. N., Howland, M. C., Sapuri-Butti, A., Rosidi, N., & Parikh, A. N. (2012). A comparison of detergent action on supported lipid monolayers and bilayers. Soft Matter, 8(14), 3734-3738. https://doi.org/10.1039/c2sm00025c

A comparison of detergent action on supported lipid monolayers and bilayers. / Ngassam, Viviane N.; Howland, Michael C.; Sapuri-Butti, Annapoorna; Rosidi, Nathan; Parikh, Atul N.

In: Soft Matter, Vol. 8, No. 14, 14.04.2012, p. 3734-3738.

Research output: Contribution to journalArticle

Ngassam, VN, Howland, MC, Sapuri-Butti, A, Rosidi, N & Parikh, AN 2012, 'A comparison of detergent action on supported lipid monolayers and bilayers', Soft Matter, vol. 8, no. 14, pp. 3734-3738. https://doi.org/10.1039/c2sm00025c
Ngassam VN, Howland MC, Sapuri-Butti A, Rosidi N, Parikh AN. A comparison of detergent action on supported lipid monolayers and bilayers. Soft Matter. 2012 Apr 14;8(14):3734-3738. https://doi.org/10.1039/c2sm00025c
Ngassam, Viviane N. ; Howland, Michael C. ; Sapuri-Butti, Annapoorna ; Rosidi, Nathan ; Parikh, Atul N. / A comparison of detergent action on supported lipid monolayers and bilayers. In: Soft Matter. 2012 ; Vol. 8, No. 14. pp. 3734-3738.
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